In this work, a Schiff base ligand namely 4-((2-(piprazine-1-yl)ethyl)pent-2-en-2-ol (HL) has been synthesized. Further the reaction between HL and NiCl₂.6H₂O salts has resulted in Schiff base complex with general formula [Ni(L)].Cl. The ligand HL and the corresponding Ni(II) complex was further characterized using various spectroscopic techniques, such as, FT-IR, ESI-MS, and elemental analysis. In the recent scenario, research in the field of protein dynamics is very crucial as these are the major part of the biological systems, and interactions of those macromolecules are responsible for various pathological diseases. Most of the metals inside the biological system shows some kind of affinity to bind with those macromolecules. Herein, it is our unmet need to address the issue by preparing some metal complexes. Therefore, fluorescence spectroscopy was introduced to check the protein binding study of BSA using Stern-Volmer equation.