In humans, cigarettes are the most common cause of toxic chemical poisoning and chemical-induced diseases. The problem of tobacco remains one of the world’s most serious problems, killing more than eight million people a year. In addition, more than 7 million deaths are due to tobacco use, while 1.2 million are non-smokers (SMS). Tobacco is consumed by 337 million Indians over a decade. According to the World Health Organization, tobacco-related deaths in India could reach 1.5 million a year by 2020. When someone smokes regularly and consistently, carboxy Hb levels rise, causing progressive hypoxia, and when CO binds to Hb, active anemia increases. This causes a decrease in tissue volume and changes in hematological parameters. Study of Lipid Profile and Thyroid Profile In Smokers and Non-Smokers The study conducted Department of Biochemistry this study included 50 healthy male subjects of age 25 -50 years Both smokers and Non-smoker subjects who were referred to S MHRC Consequently, 25 subjects were smokers and 25 subjects were non-smokers. Patients went directly to the Observed Treatment Short-course focus in the Dept. of Medicine and Dept. of Respiratory, DMMC and SMHRC, Nagpur in collaboration with JNMC & ABVRH (DMMC Deemed To Be University), Sawangi, Wardha, Maharashtra. Thyroid profile in the study group revealed a highly important difference (decrease in serum T3 and T4 and rise in serum TSH) of P<0.0001in smokers compared to non-smokers, as seen in table 1. In summary, this study will add to our understanding of how smoking affects thyroid function and will lead to our understanding of its negative effects. Because of this, it may be helpful in diagnosing and treating thyroid defects in smokers. Increased knowledge of participants about their thyroid status can serve as a smoking cessation and thus encourage them to improve their lifestyle habits. The smokers in this sample had dyslipidaemia the rate of smoking rises, as it does in heavy smokers, and the altered lipid profile worsens.