¹Rajeev Gandhi National Fellow UGC New Delhi Department of Zoology and Biotechnology, Saifia Science College, Bhopal- 462001, India

Article Publishing History

Introduction

Aloe barbadensis Miller, commonly referred to as Aloe vera, is one of more than 400 species of Aloe belonging to family Liliaceae that originated in South Africa, but have been indigenous to dry subtropical and tropical climates, including the southern USA, (Reynolds et al., 1999). Aloe vera has been used for medicinal purposes in several cultures for millennia: Greece, Egypt, India, Mexico, Japan, and China, (Foster et al., 2011). There is broad list of the therapeutic claims of different parts of Aloe vera due to its Pharmacological activities which are employed in traditional management of diverse veterinary and human diseases, (Blumenhal et al., 1998). The herb is used internally to combat most digestive problems, including constipation, poor appetite, colitis, irritable bowel syndrome as well as, asthma, diabetes, immune system enhancement, peptic ulcers, (Brusick et al., 1997; Mansour et al., 2013; Ezuruike et al., 2014; Kavyashree et al., 2015; Pandey et al., 2016).

99. verais a succulent plant. Succulents are xerophytes, Storage tissue has higher water holding capacity ranging from 99–99.5% (Hamman et al., 2008) and 0.5–1.0% solid material has over 75 different potentially active compounds including water and fat-soluble vitamins, minerals, enzymes, simple and complex polysaccharides, phenolic compounds, and organic acids. Studies on the structural components of the A. veraplant leaf portions, the rind was found to be 20–30% and the pulp 70–80% of the whole leaf weight. on a dry weight basis, the percentages of the rind and pulp represented as lipids (2.7% and 4.2%) and that as proteins (6.3% and 7.3%) only accounted for a minor fraction, (Femenia
    et al., 1999). However, the nonstarch polysaccharides and lignin were found to be 62.3% and 57.6% of the dry weight of the rind and pulp, respectively. A. vera gel polysaccharides consist of linear chains of glucose and concentrated mannose molecules, there arrangement linear chains ranging in size from a few to several thousand molecules, (Hutter et al., 1996) is referred as polymannans, (Ni Y et al., 2004; Davis et al., 2006; Chang et al., 2011; Boonyagul et al., 2014; Aranda et al., 2016).

The major polysaccharide, acemannan, repeating units of glucose and mannose in a 1:3 ratio, (Femenia et al., 1999; Ni Y. et al., 2004; Chow et al., 2005). molecular weights ranging from 30 kDa to 40 kDa. Many investigators have endeavored to establish the active principles in A. vera gel, (Habeeb et al., 2007). polysaccharides found in the inner leaf parenchymatous tissue or pulp contains proteins, lipids, amino acids, vitamins, enzymes, inorganic compounds, with different carbohydrates, (Hamman et al., 2008). Over 75 active ingredients from the inner gel have been identified, to have therapeutic effects. Different Aloe species would have varying phytochemical contents due to interspecies variation and varying climate and soil conditions, the species of Aloe selected for commercial exploitation or selected by the traditional healer, would be based on its local availability and distribution. Biological activities of a plant are due to synergistic action of the different compounds together, (Avijgan et al., 2014; Esmaeilia et al., 2015; Taukoorah et al., 2016).

Table 1: Amount of amino acids in Aloe vera gel (Mulu et al., 2015)
S. No.	Amino acid	Aloe vera (whole leaves)
1	Tryptophane	0
2	Methionine	0.02
3	Histidine	0.03
4	Cystine	0.04
5	Tyrosine	0.06
6	Isoleucine	0.07
7	Phenylalanine	0.08
8	Leucine	0.09
9	Arginine	0.12
10	Lysine	0.18
11	Proine	0.25
12	Threonine	0.33
13	Valine	0.36
14	Glutamine	0.83
15	Alanine	0.91
16	Glycine	0.95
17	Serine	1.27
18	Aspartic acid	1.75
19	Asparagines	3.29
20	Glutamic acid	4.7
21	Total concentration (nMol/mg dry mass)	15.33

In South Africa, the most widely distributed Aloe species are Aloe greatheadii var. davyana (Asphodelaceae) and Aloe ferox Mill. (Asphodelaceae). A. greatheadii grows wild in the northern parts of South Africa, whereas A. ferox grows wild primarily in the Eastern and Western Cape provinces. A. ferox contains various combinations of glucose and galactose as main monosaccharides, while A. vera yields only mannose. A. vera is rich in secondary metabolites. Anthraquinones (Aloe emodin), tricyclic aromatic quinines and chrysophanol are the major secondary metabolites, (Tan et al., 2011; Xua et al., 2016).

Table 2: Percentage of Minerals in Aloe Vera (Mulu et al., 2015)
S. No.	Minerals	Aloe vera (whole leave)
1	Iron	0.1
2	Phosphorous	0.02
3	Zinc	0.02
4	Copper	0.06
5	Magnesium	1.22
6	Calcium	3.58
7	Sodium	3.66
8	Potassium	4.06

The tricyclic aromatic quinines of Aloe were synthesized via the type III polyketide biosynthesis pathway. Recently, novel these novel plant enzymes plant-specific type III polyketide synthases (PKS), octaketide synthase, PKS4, and PKS5 were isolated from Aloe arborescens and their functions examined in E. coli, (Mizuuchi et al., 2009). their fuction is associated with biosynthesis of natural tricyclic aromatic quinines in Aloe, Aloesin, aloin and Aloe-emodin (oxidative product of aloin) are the most important secondary metabolites found in A. vera gel. Many secondary metabolites reported to have potent anti-inflammatory, lipid lowering, and antioxidant activities, (Rajasekaran et al., 2006).

Aloe is used against skin irritation, skin exposure to UV and gamma radiation, scalds, sunburn wounds, eczema, psoriasis, acne, dermatitis, ulcers, to stimulate cell regeneration. Plants have hypoglycemic, cytotoxic, antiulcer, antidiabetic effects, antibacterial, antioxidant, cardiovascular effect on human. Healing properties, anti-inflammatory, antiviral and antitumor, moisturizing, anti-aging effect, antiseptic properties of plant stimulates the immune system, only a few species of Aloe have been considered for commercial importance, of which A. vera is considered the most potent and, thereby, the most popular plant in the research field, (Surjushe et al., 2008; Davis et al., 2012; Maharijan et al., 2015; Saito et al., 2016).

Characteristics Of Aloe Vera

Physical Classification

The leaf is long triangular sheaf with two external membranes which are green and leathery. Inside this tough resistant covering is the gel, which presents itself as a compact, gelatinous mass with a translucent pearly aspect, (Vogler et al., 1999; et al., 2002; Ramachandran et al., 2012; Poor). Leaf pulp having unpleasant sensations was used to extract the juice by a decortications process. It contains some active compounds which detoxified the body stimulating property worthy of highlighting. The nutritional components of Aloe are equally distributed between the pulp and the cortex of the leaf, (Hatano et al., 2005).

Botanical Classification

The botanical genus of Aloe is Liliaceae, because it germinates from an original bulb in the same way as lilies. Tom Reynolds, a researcher from London, England, coined a new classification. In assessing the specifications and particular characteristics of the Aloe plant, they inserted it into a new botanical family, that of the Aloaceae. Aloe is a perennial evergreen shrub with succulent leaves having flowers of an elongated tubular form varying in color according to the species, from orange to bright scarlet red, particularly spectacular and reminiscent of an autumn landscape. The Aloaceae family contains approximately three hundred and fifty varieties of the plant throughout the planet.

In South Africa alone, in 1955, a total of 132 species were catalogued The range spanned from the miniature type like Aloe aristata and Aloe brevifolia, to one which can be defined as the most beautiful in existence in the world, the Aloe striata. Among the larger-sized Aloes, and those having a cosmetic, curative value, we can mention Aloe arborescens Miller, Aloe ferox, Aloe Barbadensis Miller Vera, Aloe chinensis, (Kokate et al., 2005). Aloe saponaria, and Aloe succotrine. A more generalized botanical distinction is achieved by observing the trunk and leaves.

In this way, we can distinguish three large groups of Aloe: acauleas (without a trunk), subcauleas (visible trunk but with a reduced size), (Davis et al., 1994; Das
et al., 2015; Sayed et al., 2016).

Chemical Classification

Aloe vera is a unique plant which is a rich source of many chemical compounds (Fig. I) and plays an important role in the international market. Chemistry of the plant revealed the presence of more than 200 different biologically active substances including vitamins, minerals, enzymes, sugars, anthraquinones or phenolic compounds, lignin, saponins, sterols, amino acids and salicylic acid (Chauhan et al. 2007). Studies have found that there are 75 ingredients contained in the Aloe leaf. These ingredients have a variety of medical benefits. They are divided into the following categories, (Rodriguez et al., 2005; Davis et al., 2006).

Figure 1: Aloe vera plant (Chatterjee et al., 2013)

Anthraquinones

Twelve different types of anthraquinones are present in the sap of Aloe Vera: Aloin, Isobarbaloin, Anthracene, Emodin, Ester of Cinnamonic acid, Chrysophanic acid, Barbaloin, Anthranol, Aloetic acid, Aloe Emodin, Ethereal oil and Resistannol. They act as natural laxatives, painkillers and analgesics, and they contain powerful antibacterial, antifungal and virucidal properties.

Amino Acids

Amino Acids are the building blocks of protein, which manufacture and repair muscle tissue. The human body requires 22 amino acids and needs 8 essential ones. Aloe Vera provides 20 of 22 required amino acids and 7 of 8 essential ones. These amino acids are.

Enzymes

Enzymes are natural protein molecules with highly specialized catalytic functions in biochemical reactions produced by all living organisms (microorganisms, plants, animals, and human beings). Although like all other proteins, enzymes are composed of amino acids, they differ in function in that they have the unique ability to facilitate biochemical reactions without undergoing change themselves. Some of the most important enzymes in Aloe Vera are: Peroxidase, Aliiase, Catalase, Lipase, Cellulose, Carboxypeptidase, Amylase and Alkaline Phosphates.

Vitamins

Aloe Vera contains numerous vitamins: Vitamins A, C, & E (crucial antioxidants that combat dangerous free radicals in the body). Vitamin B & Choline (concerned with the production of energy, amino acid metabolism and developing muscle mass). Vitamin B12 (responsible for the production of red blood cells) and Folic acid (helps develop new blood cells).

Minerals

Aloe Vera contains the following minerals: Calcium: (essential for proper bone and teeth density). Manganese: (a component of enzymes necessary for the activation of other enzymes). Sodium: (ensures that the body fluids do not become too acidic or too alkaline). Copper: (enables iron to work as oxygen carriers in the red blood cells). Magnesium: (used by nerves and muscle membranes to help conduct electrical impulses). Potassium: (regulates the acidic or alkaline levels of body fluid). Zinc: (contributes to the metabolism of proteins, carbohydrates and fats), Chromium: (necessary for the proper function of insulin, which in turn controls the sugar levels in the blood). Iron: (controls the transportation of oxygen around the body via the red blood
cells.

Lignin

This cellulose substance is found in the gel has no known medical properties except it posses the property of penetrating the human skin.

Saponins

These form soapy lathers when mixed and agitated with water. They have been used in detergents, foaming agents and contain antiseptic properties.

Sugars

Aloe Vera contains both monosaccharide, such as glucose and fructose, and polysaccharides. Polysaccharides are the most important types of sugars. They aid in proper digestion, maintain cholesterol levels, improve liver functions and promote the strengthening of
bones.

Sterols

Sterols are important anti-inflammatory agents. The ones found in Aloe Vera are: Cholesterol, Sitosterol, Campesterol and Lupeol. These sterols contain antiseptic and analgesic properties. They also have pain killing properties similar to aspirin.

Therapeutic Uses With
Mechanism Of Action

Wound Healing

The wound healing property of Aloe vera gel has been attributed to Mannose-6-phosphate used for first to second degree burns, (Davis et al., 1994; Maenthaisong et al., 2007).Wound healing is a dynamic process, occurring in 3 phases. The first phase is inflammation, hyperaemia and leukocyte infiltration. The second phase consists of removal of dead tissue. The third phase of proliferation consists of epithelial regeneration and formation of fibrous tissue, (Reddy et al., 2011).

Glucomannan and plant growth hormone gibberellin interacts with growth factor receptors of fibrobroblast and stimulate its activity and proliferation for increased collagen synthesis in topical and oral administration of Aloe, (Hayes et al., 1999). The Aloe gel has been used for the treatment of radiation burns and radiation ulcers, (Syed et al., 1997) and complete healing has been observed in two radiation burns patients, (Yeh et al., 2003).

The fresh gel was more effective than the cream, (Visuthikosol et al., 1995) as Aloe gel treated lesions healed faster (11.8 days) compared to burns treated with petroleum jelly gauze (18.2 days) by Fulton, (Fulton et al., 1990). The 27 patients with partial thickness burns have been treated with Aloe gel in a placebo-controlled study, (Montaner et al., 1996). The Aloe administration influence collagen composition (more type III) and increased collagen cross linking for wound contraction and improving breaking strength, (Reynolds et al., 1999). It also increases synthesis of hyaluronic acid and dermatan sulfate in the granulation tissue of a healing wound, (Chithra et al., 1998 Joshy et al.,
2016).

Anti-Inflammatory Action

In vitro and In vivo studies of Aloe vera gel in experimental model shows the anti-inflammatory activity due to bradykinase activity, (Che et al., 1991; Tyler et al., 1994). The peptidase bradykinase was isolated from aloe break down the bradykinin, an inflammatory substance that induces pain, (Ito et al., 1993).

A novel anti-inflammatory compound, C-glucosyl chromone, was isolated from gel extracts, (Haller et al., 1990). Aloe vera inhibits the cyclo-oxygenase pathway and reduces prostaglandin E2 production from arachidonic acid. Fresh Aloe vera gel significantly reduced acute inflammation in rats (carrageenin-induced paw oedema), but not in chronic inflammation, (Che et al., 1991).

The aloe sterol includes campesterol, â-sitosterol, lupeol, and cholesterol which are anti-inflammatory in nature, helps in reducing the inflammation pain and act as a natural analgesic. Other aspirin like compound present in Aloe is responsible for anti-inflammatory and antimicrobial properties, (Sydiskis et al., 1991; Ferro et al., 2003; Agarry et al., 2005). Even, Aloe vera extract (5.0% leaf homogenate) decreased inflammation by 48% in a rat adjuvant-induced arthritic inflammatory model, (Hanley et al., 1982; Davis et al., 1991).

Three Aloe vera gel sterols were able to reduce inflammation by up to 37%. Lupeol, the most active antiinflamatory sterol, reduced inflammation in a dose dependent manner. The data suggest that specific plant sterols may also contribute to the anti-inflammatory activity of gel, (Haller et al., 1990; Jensen et al., 2015).

Effects On The Immune System

In a study on mice that had previously been implanted with murine sarcoma cells, acemannan stimulates the synthesis and release of interleukin-1 (IL-1) and tumor necrosis factor from macrophages in mice, which in turn initiated an immune attack that resulted in necrosis and regression of the cancerous cells, (Peng et al., 1991). Alprogen inhibit calcium influx into mast cells, thereby inhibiting the antigen-antibody-mediated release of histamine and leukotriene from mast cells, (Hansel et al., 1994).

Several low-molecular-weight compounds are also capable of inhibiting the release of reactive oxygen free radicals from activated human neutrophils, (Hart et al., 1990; Lee et al., 2001; Zanuzzo et al., 2015).

Moisturizing And Anti-Aging

Muco-polysaccharides of Aloe vera has Agent which help in binding moisture into the skin. The amino acids also soften hardened skin cells and zinc acts as an astringent to tighten pores. Its moisturizing effects have also been studied in treatment of dry skin associated with occupational exposure where Aloe vera gel gloves improved the skin integrity, decrease appearance of acne wrinkle and decrease erythema, (West et al., 2003).

The Aloe gel gives cooling effect and also acts as a moisturizing agent. It also has role in gerontology and rejuvenation of aging skin. This property of Aloe is because it’s biogenic material. Aloe vera is used as skin tonic in cosmetic industry, (Kaushik et al., 2016)

Antitumor Activity

Glycoproteins present in Aloe vera gel have been reported to have antitumor and antiulcer effects and to increase proliferation of normal human dermal cells, (Yagi et al., 1997; Choi et al., 2001; Yagi et al., 2003). In recent studies, a polysaccharide fraction has shown to inhibit the binding of benzopyrene to primary rat hepatocytes, thereby pre- venting the formation of potentially cancer-initiating benzopyrene DNA adducts. An induction of glutathione S-transferase and an inhibition of the tumor-promoting effects of phorbol myristic acetate has also been reported which suggest a possible benefit of using aloe gel in cancer chemoprevention, (Kim et al., 1997; 1999; Yonehara et al., 2015).

Laxative Effects

Anthraquinones present in latex are a potent laxative; its stimulating mucus secretion, increase intestinal water content and intestinal peristalsis, (Ishii et al., 1994). The laxative effect of Aloe is not generally observed before 6 hours after oral administration, and sometimes not until 24 or more hours after. The Aloe effects are primarily due to the 1, 8-dihydroxyanthracene glycosides, Aloin A and B (formerly designated barbaloin) (Tyler et al., 1988; 1994).

After oral administration Aloin A and B, which are not absorbed in the upper intestine, are hydrolysed in the colon by intestinal bacteria and then reduced to the active metabolites (the main active metabolite is aloe-emodin-9-anthrone), (Che et al., 1991), which like senna acts as a stimulant and irritant to the gastrointestinal tract. Aloe latex is known for its laxative properties, (Reynolds et al., 1993 Haque et al., 2014).

Medicinal Uses

Aloe vera is anthelmintic, aperients, carminative, deobstruent, depurative, diuretic, stomachic and emmenagogue. Juice is used in skin care medicine, dyspepsia, amenorrhea, burns, colic, hyperadenosis, hepatopathy, splenopathy, constipation, span menorrhea, abdominal tumors, dropsy carbuncles, sciatica, lumbago and flatulence. It is used in ayurvedic formulations as appetite-stimulant, purgative, emmenogogue and antihelminthic, for treating cough, colds, piles, debility, dyspnoea, asthma and jaundice, (Joseph et al., 2010).

Traditionally, Aloe vera gel is used both, topically (treatment of wounds, minor burns, and skin irritations) and internally to treat constipation, coughs, ulcers, diabetes, headaches, arthritis, immune-system deficiencies, (Vogler et al., 1999; Eshun et al., 2004). The bioactive compounds are used as astringent, haemostatic, antidiabetic, antiulcer, antiseptic, antibacterial, anti inflammatory, antioxidant and anticancer agent also, effective in treating stomach ailments, gastrointestinal problems, skin diseases, constipation, radiation injury, wound healing, burns, dysentery, diarrhoea and in the treatment of skin diseases, (Baker et al., 1975; Ali SA et al., 2012).

The Egyptians used the Aloe vera to make papyrus like scrolls as well as for treatment of tuberculosis, (Nadkerni et al., 1976). Nadkerni stated various preparations of Aloe barbadensis like confection, lotion and juice, useful remedies for curing various diseases. Aloe contains mixture of glucosides collectively called aloin which is the active constituent of various drugs. Traditionally Aloe is extensively used in treating urine related problems, pimples and ulcers etc. It is also used in gerontology and rejuvenation of aging skin.

The juice of Aloe vera leaves is used as stomachic tonic and purgative. Scientific evidence for the cosmetic and therapeutic effectiveness of Aloe vera is limited and when present is frequently contradictory, (Ernst et al., 1998; Marshall et al., 2000). Despite this, the cosmetic and alternative medicine industries regularly make claims regarding the soothing, moisturizing, and healing properties of Aloe vera, especially via internet advertising, (Kunkel et al., 1984; Boudreau et al., 2006, Sahu
et al., 2013, Figure 3).

Figure 2: Components of Aloe vera (Chauhan et al., 2007)

Figure 3: Representing the medicinal utilities of Aloe vera (Sahu et al., 2013)

Cosmetic & Skin Protection Application

Aloin and its gel are used as skin tonic against pimples. Aloe vera is also used for soothing the skin, and keeping the skin moist to help avoid flaky scalp and skin in harsh and dry weather. The Aloe sugars are also used in moisturizing preparations, (Barcroft et al., 2003). Mixed with selected essential oils, it makes an excellent skin smoothening moisturizer, sun block lotion plus a whole range of beauty products.

Due to its soothing and cooling qualities, Aloe vera extracts have antibacterial and antifungal activities, which may help in the treatment of minor skin infections It reduces the production and release of skin keratinocyte derived immunosuppressive cytokines such as interleukin-10 (IL-10) and hence prevents UV-induced suppression of delayed type hypersensitivity, (Byeon
et al., 1988).

Aloe vera gel has an antioxidant protein, metallothionein, is generated in the skin, which scavenges hydroxyl radicals and prevents suppression of superoxide dismutase and glutathione peroxidase in the skin. Skin burns effect is reported and radiation dermatitis, (Shelton et al.,1991; Visuthikosol et al. 1995; Bosley et al., 2003; Vastrad et al., 2015).

Antiseptic

The antiseptic property of Aloe vera is due to presence of six antiseptic agents namely lupeol, salicylic acid, urea nitrogen, cinnamonic acid, phenols and sulphur. These compounds have inhibitory action on fungi, bacteria and viruses, (Zawahry et al., 1973; Qian et al., 2015; Anjum et al., 2016).

Anti Diabetic

Reviewed the beneficial effects of selective medicinal plant species such as Allium cepa, Allium sativum, Aloe vera, Azadirachta indica, Gymnema sylvestre, Syzygium cumini and Pterocarpus marsupium, and emphasize on the role of active bio-molecules which possess anti-diabetic activity. The five phytosterols of A. vera, lophenol, 24-methyl- lophenol, 24-ethyl-lophenol, cycloartanol and 24-methy- lenecycloartanol showed anti-diabetic effects in type-2 diabetic mice, (Tanaka, et al., 2006; Noor et al., 2008). Traditional anti-diabetic plants might provide new oral anti-diabetic compounds, which can counter the high cost and poor availability of the current medicines for many rural populations in developing countries.

Extracts of Aloe gum increases glucose tolerance in both normal and diabetic rats, (Awadi et al.,1987) and Aloe vera sap taken for 4 – 14 weeks has shown a significant hypoglycaemic effect both clinically and experimentally, (Ghannam et al., 1986). The treatment of diabetes mellitus has been attempted with various indigenous plants and polyherbal formulations, (Chaurasia
et al., 1994; Mitra et al., 1996). Aloe vera contains polysaccharides which increase the insulin level and show hypoglycemic properties, (Yagi et al., 2006; Epifanoa
et al., 2015; Muammar et al., 2016).

Anticancer Properties

Aloe vera juice enables the body to heal itself from cancer and also from the damage caused by radio and chemotherapy that destroys healthy immune cells crucial for the recovery. Aloe vera emodin, an anthraquinone, has the ability to suppress or inhibit the growth of malignant cancer cells making it to have antineoplastic properties, (Thomson et al., 1971). The role of Aloe in carcinogenicity has not been evaluated well. The chronic abuse of anthranoid-containing laxatives has been hypothesized to play a role in colorectal cancer, and (Siegers et al., 1992; 1993). Aloe vera tincture and melatonin administration was studied as standard therapy against metastatic solid tumors, (Furukawa et al., 1991; Fenig et al., 2004; Shalabia et al., 2015; Chouhan et al., 2016).

Stress

Aloe vera is an excellent example of a functional food that plays a significant role in protection from oxidative stress, (Barcroft et al., 2003; Joseph et al., 2010). Aloe juice is helpful in smooth functioning of the body machinery, (Saroj et al., 2004). It reduces cell-damaging process during stress condition and minimizes biochemical and physiological changes in the body, (Foster et al., 1999).

Oxidative stress refers to chemical reactions in which compounds have their oxidative state changed. Some antioxidants are part of the body’s natural regulating machinery while other dietary antioxidants are derived from diet sources, (Kaithwasa et al., 2014; Zhou et al., 2015; Cartera et al., 2016).

Reduces Inflammation

It improves joint flexibility and helps in the regeneration of body cells. It strengthens joint muscles, which therefore reduces pain and inflammation in weakened or aged joints. (Sahgal et al., 2015; Susman et al., 2016)

Increases Energy Levels

Our diets include many substances which can cause fatigue and exhaustion. Taken regularly, Aloe vera juice ensures a greater feeling of well-being, allowing energy levels to increase and also helps maintain a healthy body weight, (Lad et al., 2013; Kumbhar et al., 2015; Pani et al., 2016)

BUILDS IMMUNITY

It is especially great for those who have chronic immune disorders like polysaccharides or fibromyalgia since the polysaccharides in Aloe vera juice stimulate macrophages, the white blood cells that fight viruses, (Hart et al., 1989; Turner et al., 2004; Algieri et al., 2015; Shokri et al., 2016).

Detoxifies

Aloe vera juice is a great natural aid to detox. With our stressful lives, the pollution around us and the junk foods we eat, we all need to cleanse our systems from time to time. Drinking Aloe vera juice provides a fantastically rich cocktail of vitamins, minerals and trace elements to help our bodies deal with these stresses and strains every day, (Chaturved et al., 2007; Cellini et al., 2014; Lee et al., 2015; Mehta et al., 2016)

Aloe Vera Protects Aluminium Induced Changes In Brain Enzyme Activity

Aluminium sulphate has significantly altered the normal levels of acetyl cholinesterase, sodium potassium ATPase and glutathione of rat brain. The levels of brain enzymes were found to be highly decreased in both the alumiuium treated groups. But in contrast to this, elevated levels of acetyl cholinesterase, sodium potassium ATPase and glutathione were noticed in aloin and aluminium sulphate co treated groups, indicating the protective role of aloin against aluminium sulphate toxicity, (Jakkala and Ali 2015).

Amelioration Of Histopathological Damages In Testis Of Albino Rats By Aloe

Aloin has been recently found to ameliorate the toxic effects of aluminium sulphate, the histological structures of the seminiferous tubules and their cell cytoplasm, nuclei, nutritive cells, which were found to become near normal by treatment of aloin for more than 30 days, (Miraj et al., 2014; Jakkala and Ali 2016).

Antimicrobial Activities

Antibacterial Activity

The Aloe extract was potent against three strains of Mycobacterium (M. fortuitum, M. smeg- matis and M. kansasii) and a strong anti-mycobacterial activity against M. tuberculosis ss well as antibacterial activity against P. aeruginosa, E. coli, S. aureus and S. typhi. Streptoccocus pyogenes and Streptococcus faecalis are two microorganisms that have been inhibited by Aloe vera gel. Glucomannan and acemannan have been proved to accelerate wound healing, activating macrophages, stimulating immune system as well antibacterial and antiviral effects, (Davis et al., 1987; 1988; Kaufman et al., 1989; Visuthikosol et al., 1995; Djeraba
et al., 2000).

Aloe vera gel was bactericidal against Pseudomonas aeruginosa and acemannan prevented it from adhering to human lung epithelial cells in a monolayer culture, a processed Aloe vera gel preparation inhibited the growth of fungus Candida albicans, (Heggers et al., 1979; Stanley et al., 2014; Kumar et al., 2015; Rezazadeh et al., 2016).

Antiviral Activity

Electron micrograph examination of anthro-quinone treated herpes simplex virus demonstrated that the envelopes were partially disrupted. Such results indicate that anthraquinones extract from variety of plants are directly virucidal to enveloped viruses.. The anthraquinone aloin also inactivates various enveloped viruses such as herpes simplex, varicella zoster and influenza, (Sydiskis et al., 1991). Several ingredients in Aloe vera gel have been shown to be effective antiviral agent. acemannan reduced herpes simplex infection in two cultured target cell lines, (Kemp et al., 1990).

Lectins, fractions of Aloe vera gel, directly inhibited the cytomegalovirus proliferation in cell culture, perhaps by interfering with protein synthesis. A purified sample of aloe emodin was effective against infectivity of herpes simplex virus Type I and Type II and it was capable of inactivating all of the viruses, including varicella zoster virus, influenza virus, and pseudorabies virus, (Jia et al., 2008; Silvaa et al., 2014; Sadeghi et al.,2015; Rezazadeh et al., 2016).

Antifungal Activity

Aloe vera was evaluated on the mycellium development of Rhizoctonia solani, Fusarium oxysporum, and Colle- totrichum coccodes, that showed an inhibitory effect of the pulp of A. vera on F. Oxysporum, R. solani reduced the rate of colony growth, (Cheesbrough et al.,1984; Rodríguez et al.,2005), (Agarry et al., 2005). reported that the Aloe gel inhibited the growth of Trichophyton mentagrophytes, while the leaf possesses inhibitory effects on both Pseudomonas aeruginosa and Candida albicans. In contrast, Aloe vera extracts failed to show antibiotic proper ties against Xanthomonas species, (Satish et al., 1999). The saponins perform strongly as anti-microbial against bacteria, viruses, fungi and yeasts, (Joerger et al., 2001; Peter et al., 2002; Moghadamtousi et al., 2014; Shireen et al., 2015; Ahmed et al., 2016).

Adverse Reactions

Abdominal spasms and pain may occur after even a single dose and overdose can lead to colicky abdominal spasms and pain, as well as the formation of thin, watery stools. Chronic abuse of anthraquinone stimulant laxatives can lead to hepatitis, (Beuers et al., 1991) and electrolyte disturbances (hypokalaemia, hypocalcaemia), metabolic acidosis, malabsorption, weight loss, albuminuria, and haematuria, (Godding et al., 1976; Muller et al., 1993).

Secondary aldosteronism may occur owing to renal tubular damage after aggravated use. Weakness and orthostatic hypotension may be exacerbated in elderly patients when stimulant laxatives are repeatedly used, (Rockville et al., 1992). Steatorrhoea and protein-losing gastroenteropathy with hypoalbuminaemia have also been observed, as have excessive excretion of calcium in the stools and osteomalacia of the vertebral column, (Heizer et al., 1968; Ernst et al., 2000; Hajheydari et al., 2014; Miroddi et al., 2015; et al., 2016).

Side Effects

Topical

It may cause redness, burning, stinging sensation and rarely generalized dermatitis in sensitive individuals. Allergic reactions are mostly due to anthraquinones, such as aloin and barbaloin. It is best to apply it to a small area first to test for possible allergic reaction, (Davis et al., 1989; Heggers et al., 1995; Dhikav et al., 2002; Sharma et al., 2014; Ahluwaliaa et al.,2016).

Oral

Abdominal cramps, diarrhoea, red urine, hepatitis, dependency or worsening of constipation. Prolonged use has been reported to increase the risk of colorectal cancer. Laxative effect may cause electrolyte imbalances (low potassium levels), (Chinnusamy et al., 2009; Salazar et al., 2010; Sundarkar et al., 2011; Kareman et al., 2013; Akev et al., 2015; Ahmada et al.,2016).

Conclusion And Future Aspects

In recent years, ethnobotanical and traditional uses of natural compounds, especially of plant origin received much attention as they are well tested for their efficacy and generally believed to be safe for human use. They obviously deserve scrutiny on modern scientific lines such as physiochemical characterization, biological evaluation, toxicity studies, investigation of molecular mechanism of action of isolated phytoprinciple and their clinical trials. These are necessary classical approaches in search of new lead molecule for management of various diseases.

Many Indian herbs are being used in traditional practices to cure various human ailments. Aloe vera, has an important place among such wound healing medicinal plants, it can also be used in treating inflammation, pain, ulcer and antihyperglycaemic agent. Furthermore, in future study, the isolated principles from Aloe vera needs to be evaluated in scientific manner using various innovative experimental models and clinical trials to understand its mechanism of action, in search of other active constituents, so that its other therapeutic uses can be widely explored.

Aloe vera is a medicinal plant and due to its extensive medicinal, nutraceutical and other uses it’s enjoy a great demand in the market across the globe. The major markets for Aloe vera and its extracts are Australia, US and the entire Europe. Given the exponentially growing demand for it in the international market, Aloe vera presents the finest commercial opportunity among the various medicinal plants. India is among the few countries gifted with the unique geographical features essential for cultivation of Aloe vera and other high potential medicinal plants.

Acknowledgements

Authors are thankful to UGC, New Delhi for providing financial support for research work. We are also thankful to Principal and Secretary, Saifia Science College, Bhopal for providing necessary facilities and support.

References

Agarry O. O., Olaleye M. T. and Bello-Michael C. O. (2005).Comparative Antimicrobial Activities of Aloe vera Gel and Leaf. African Journal of Biotechnology, Vol. 4, No. 12, pp. 1413-1414.

Ahluwaliaa B, Magnussona M.K., Isakssona S., Larssonc, F., Ohmana L. (2016). Effects of Aloe barbadensis Mill. extract (AVH200 ®) on human blood T cell activity in vitro. Journal of Ethnopharmacology Vol. 179, (17); pp. 301–309.

Ahmada M., Khana Z., Mukhtara A., Zafara M., Sultanaa S., Jahanb S. (2016). Ethnopharmacological survey on medicinal plants used in herbal drinks among the traditional communities of Pakistan. Journal of Ethnopharmacology Vol.184, 26 May, pp.154–186.

Ahmed S., Ahmad M., Swami B.L., Ikram S. (2016). A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. Journal of Advanced Research Volume 7, Issue 1, PP.17–28.

Al-Awadi F. M., and Gumaa K. A. (1987). Studies on the Acti- vity of Individual Plants of an Antidiabetic Plant Mixture, Acta Diabetologica Latina, Vol. 24, No. 1, pp. 37-41.

Ali SA, Galgut JM, Choudhary RK. (2012). on the novel action of melanolysis by a leaf extract of Aloe vera and its active ingredient aloin, potent skin depigmenting agents. Planta Med.78(8):767-71.

Akev N., Can A., Sutlupınar N., Çandoken E., Ozsoy N., Ozden T.Y.,Yanardag R.,Uzen E. (2015). Twenty years of research on Aloe vera. Istanbul Ecz. Fak. Derg. / J. Fac. Pharm. Istanbul 45(2); pp.191-215.

Algieri F., Nogales AR., Cabezas MER., Risco S., Ocete M. A., Galvez J. (2015).Botanical Drugs as an Emerging Strategy in Inflammatory Bowel Disease: A Review. Mediators of Inflammation. Article ID 179616, p14.

Anjum S., Gupta A., Sharma D., Gautam D., Bhan S., Sharma A., Kapil A., Gupta B.(2016). Development of novel wound care systems based on nanosilver nanohydrogels of polymethacrylic acid with Aloe vera and curcumin. Materials Science and Engineering.

Aranda Cuevas, B., Herrera Méndez, C., Flores, I. , Solís-Pereira, S. , Cuevas-Glory, L. , Muñoz, G. , Vargas y Vargas, M. and Cortez, J. (2016). Main Polysaccharides Isolated and Quantified of Aloe vera Gel in Different Seasons of the Year. Food and Nutrition Sciences, 7, 447-453.

Avijgan M., Mahboubi M., Moheb Nasab M., Ahmadi Nia E., Yousefi H. (2014). Synergistic activity between Echinophora platyloba DC ethanolic extract and azole drugs against clinical isolates of Candida albicans from women suffering chronic recurrent vaginitis. J Mycol Med. ;24:112–116.

Baker O.T. (1975). The Amazing Ancient to Modern Useful Plant Aloe vera: Amazing Plant of the Magic Valley, R. Prevost, Lemon Grove.

Barcroft and Myskja, (2003). Aloe vera: Nature’s Silent Healer, BAAM.

Beuers, U., Spengler U., and Pape G.R. (1991). Hepatitis after Chronic Abuse of Senna, Lancet, Vol. 337, No. 8737,
pp. 472.

Blumenhal M., Busse N.R. and Golddberg A. (1998). Boson M.A. Integrative Medicines Communications, 80-81.

Bosley C., Smith J., Baratti P. (2003). A Phase III Trial Comparing an Anionic Phospholipidbased (APP) Cream and Aloe vera-Based Gel in the Prevention and Treatment of Radiation Dermatitis, International Journal of Radiation Oncology Biology Physics, Vol. 57, No. 2,pp. 34-38.

Boudreau M. D. and Beland F. A. (2006). An Evaluation of the Biological and Toxicological Properties of Aloe barbadensis (Miller), Aloe vera, Journal of Environmental Science and Health, Vol. 24, pp. 103-154.

Brusick D. and Mengs V. (1997). Environ. Molec. Mutag., 29, 1-9.

Byeon S., Pelley R., Ullrich S. E., Waller T. A., Bucana C. D., Strickland F. M. (1988). Aloe barbadensis Extracts Reduce the Production of Interleukin-10 after Exposure to Ultraviolet Radiation, Journal of Investigative Dermatology, Vol. 110, pp. 811-817.

Chaturvedi N (2007). The burden of diabetes and its complications: trends and implications for intervention.Diabetes Res Clin Pract 76: S3-12.

Chaurasia A. K., Dubey S. O., Ojha J. K. (1994). Role of Vijaysara and Jarul on Insulin Dependent Diabetes Mellitus, Aryavaidyan, Vol. 7, No. 3, pp. 147-152.

Che Q. M., Akao T., Hattori M., Kobashi K., Namba T. (1991). Isolation of Human Intestinal Bacteria Capable of Transforming Barbaloin to Aloe-Emodin Anthrone, Planta Medica, Vol. 57, No. 1, pp. 15-19.

Cheesbrough M. (1984). Medical Laboratory Manual for Tropical Countries, The University Press, Cambridge.

Chinnusamy K., Nandagopal T., Nagaraj K., Sridharanet S. (2009). Aloe vera induced oral mucositis: A case report. Internet J Pediatr Neonatol;9(2).

Chang X.L., Feng Y.M. and Wang W.H. (2011). Comparison of the Polysaccharides Isolated from Skin Juice, Gel Juice and Flower of Aloe arborescens Tissues. Journal of the Taiwan Institute of Chemical Engineers, 42, 13-19.

Chatterjee P., Chakraborty B., and Nandy S.(2013). Aloe vera plant: review with significant pharmacological activities. Mintage journal of Pharmaceutical & Medical Sciences,21-24.

Chouhan A., Karma A., Artani N., Parihar D. (2016).Overview on cancer: role of medicinal plants in its treatment. World Journal of Pharm and pharmaceutical Sci. Vol. 5, Issue 5, pp.185-207.

Chithra P. G., Sajithal B., Chandrakasan G. (1998). Influence of Aloe vera on Glycosaminoglycans in the Matrix of Healing Dermal Wounds in Rats, Journal of Ethanopharmacology, Vol. 59, No. 3, pp. 179-186.

Choi S. W., Son B. W., Son Y. S., Park Y. I., Lee S. K., Chung M. H. (2001). The Wound Healing Effect of a Glycoprotein Fraction Isolated from Aloe vera, British Jour- nal of Dermatology, Vol. 145, No. 4, pp. 535-545.

Chow J.T.-N., Williamson D.A., Yates K.M., Goux W.J. (2005). Chemical characterization of the immunomodulating polysaccharide of Aloe vera L. Carbohydr Res.;340:1131–1142.

Davis R. H., Kabbani J. M., and Maro N. P. (1987). Aloe vera and Wound Healing, Journal of the American Podiatric Medical Association, Vol. 77, No. 4, pp. 165-169.

Davis R. H., Leitner M. G., and Russo J. M. (1988). Aloe vera. A Natural Approach for Treating Wounds, Edema, and Pain in Diabetes, Journal of the American Podiatric Medical Association, Vol. 78, No. 2, pp. 60-68.

Davis R. H., Leitner M. G., Russo J. M. and Byrne M. E. (1989). Wound Healing. Oral and Topical Activity of Aloe vera, Journal of the American Paediatric Medical Association, Vol. 79, No. 11, pp. 559-562.

Davis R. H., Parker W. L., Samson R. T., Murdoch D. P. (1991). Isolation of a Stimulatory System in an Aloe Extract, Journal of the American Podiatric Medical Asso- ciation, Vol. 81, pp. 473-478.

Davis R H., Agnew PS. (2006). Antiarthritic activity of anthraquinones found in Aloe for podiatric medicine. J Am.Pod. Med. Assn. 76:2:61-66.

Davis RH, Agnew PS, (2006). Antiarthritic activity of anthraquinones found in Aloe for podiatric medicine. J Am.Pod. Med. Assn. 76: 2: 61-66.

Das A., Haque S.K.M., Ghosh B., Nandagopal K., Jha T.B. (2015). Morphological and Genetic Characterization of Micropropagated Field Grown Plants of Aloe vera L. Plant Tissue Cult. & Biotech. 25(2): 231-246.

Davis, R. H., Di Donato J. J., Hartman G. M., Hass, R. C. (1994). Anti-Inflammatory and Wound Healing Activity of a Growth Substance in Aloe vera, Journal of the American Podiatric Medical Association, Vol. 84, No. 2, pp. 77-81.

Dhikav V., Singh S., and Anand K. S. (2002). Newer non-steroidal anti-inflammatory drugs-a review of their therapeutic potential and adverse drug reactions, Journal of the Indian Academy of Clinical Medicine, vol. 3, no. 4, pp. 332–338.

Djeraba A., and Quere P. (2000). In Vivo Macrophage Activation in Chickens with Acemannan, a Complex Carbohydrate Extracted from Aloe vera, International Journal of Immunopharmacology, Vol. 22, No. 5, pp. 365-372.

Drury R.A.B. and Wallington E.A. (1980). Carleton’s histological technique. 5th ed. Oxford,

Ernst E. (2000). Adverse effects of herbal drugs in dermatology. Br J Dermatol 143, pp. 923-929.

Epifanoa F., Fioritoa S., Locatellia M., Taddeoa VA., Genovesea S. (2015). Screening for novel plant sources of prenyloxyanthraquinones: Senna alexandrina Mill. and Aloe vera (L.) Burm. F. Natural Product Research: Formerly Natural Product Letters. Volume 29, (2); p. 180-184.

Esmaeilia A., and Ebrahimzadeh M. (2015). Polymer-Based of Extract-Loaded Nanocapsules Aloe vera L. Delivery. Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry.Vol. 45, Issue 1, pp. 40-47.

Ezuruike U.F., Prieto J.M. (2014).The use of plants in the traditional management of diabetes in Nigeria: Pharmacological and toxicological considerations. Journal of Ethnopharmacology Vol. 155, Issue 2, (11); pp 857–924.

Ernst E., and Fugh B. A. (1998). Methodological Considerations in Testing the Efficacy of Complementary/Al- ternative Treatments (CATs), The Journal of Alternative and Complementary Medicine, Vol. 16, pp. 8-10.

Eshun K. and He, Q. (2004). Aloe vera: A Valuable Ingredient for the Food, Pharmaceutical and Cosmetic Industries—A Review, Critical Reviews in Food Science and Nutrition, Vol. 44, No. 2, pp. 91-96.

Femenia A., Sanchez E.S., Simal S., Rossello C. (1999). Compositional features of polysaccharides from Aloe vera (Aloe barbadensis Miller) plant tissues. Carbohydr Polym. ;39:109–
117.

Fenig E., Nordenberg J., Beery E., J. Sulkes and Wasserman L. (2004). Combined Effect of Aloe-Emodin and Chemotherapeutic Agents on the Proliferation of an Adherent Variant Cell Line of Merkel Cell Carcinoma, Oncology Reports, Vol. 11, No. 1, pp. 213-217.

Ferro V.A., Bradbury F., Cameron P., E., Shakir S., Rahman R., Stimson W. H. (2003). In Vitro Susceptibilities of Shigella flexneri and Streptococcus pyogenes to Inner Gel of Aloe barbadensis Miller, Antimicrobial Agents and Chemotherapy, Vol. 47, No. 3,pp. 1137-1139.

Foster M., Hunter D., Samman S. (2011). Evaluation of the nutritional and metabolic effects of Aloe vera. In: Benzie I.F.F., Wachtel-Galor S., editors. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd ed. CRC; Boca Raton.

Foster S. (1999). Aloe vera: The Succulent with Skin Soothing Cell Protecting Properties, Herbs for Health Magazine.

Fulton J. E. (1990). The Stimulation of Postdermal Abarasion Wound Healing with Stabilised Aloe vera Gel-Poly- thylene Oxide Dressing, Journal of Dermatologic Sur- gery & Oncology, Vol. 16, No. 5, pp. 460-467.

Furukawa F., Nishikawa A., T., Chihara K. Shimpo and Beppu H. (1991). Inactivation of Enveloped Viruses by Anthra- quinones Extracted from Plants, Antimicrobial Agents and Chemotherapy, Vol. 35, No. 12, pp. 2463-2466.

Ghannam N., Kingston M., I. Al-Meshaal A., Tariq M., Parman N. S., Woodhouse N. (1986). The Antidiabetic Activity of Aloes: Preliminary Clinical and Experimental Obser- vations, Hormone Research, Vol. 24, No. 4, pp. 286-
294.

Ghannam N., Kingstn and Al-Meshall I.A. (1986). The antidiabetic activity of aloes: preliminary clinical and experimental observations. Horm. Res. 24, 288-294.

Gilmar G.A., Rall W.T., Nies S.A. and Taylor P. (1992). Pharmacological Basics of Therapeutics, II. English edition. McCrow-Hill International Editions Medical Series.

Godding E. W. (1976). Therapeutics of Laxative Agents with Special Reference to the Anthraquinones, Pharmacology, Vol. 14, No. 1, pp. 78-101.

Habeeb F., Shakir E., Bradbury F. (2007). Screening methods used to determine the anti-microbial properties of Aloe vera inner gel. Methods;42:315–320.

Haller J. S. (1990). A Drug for All Seasons, Medical and Phar- macological History of Aloe, Bulletin of the New York Academy of Medicine, Vol. 66, pp. 647-659.

Haller J. S. (1990). A Drug for All Seasons, Medical and Pharmacological History of Aloe, Bulletin of the New York Academy of Medicine, Vol. 66, pp. 647-659.

Hamman J.H. (2008). Composition and applications of Aloe vera leaf gel Molecules pp. 1599–1616.

Hanley D. C., Solomon W. A., Saffran B., Davis R. H. (1982). The Evaluation of Natural Substances in the Treatment of Adjuvant Arthritis, Journal of the American Podiatric Medical Association, Vol. 72, pp. 275-284.

Hansel R., Keller K., Rimpler H., Schneider G. (1994). Hagers Handbuch der Pharmazeutischen Praxis. Monograph: Valeriana, 5th Edition, Springer, Berlin.

Hart L. A., Nibbering P. H., van M. T., den Barselaar H., van Dijk A. J., Burg van den., Labadie R. P. (1990). Effects of Low Molecular Constituents from Aloe vera Gel on Oxidative Metabolism and Cytotoxic and Bactericidal Activities of Human Neutrophils, International Journal of Immunopharmacology, Vol. 12, No. 4, , pp. 427- 434.

Hart L.A, Berg V.D.,Kuis L, Dijk H, (1989).Labadie R.P. An anti­complementary poly­saccharide with immunological adjuvant activity from the leaf parenchyma gel of Aloe vera. Planta Med. 55:509–12.

Hatano T. (2005). Effects of tannins and related polyphenols on methicillin-resistant Staphylodcoccus aureus. Phytochemistry 66:2047-2055.

Hayes S. M. (1999). Lichen Planus: Report of Successful Treatment with Aloe vera, General Dentistry, Vol. 47, No. 3, pp. 268-272.

Heggers J. P., Kucukcelibi A., Stabenou C. J., Ko F., Broemeling L. D., Robson M. C. and Winters W. D. (1995). Wound Healing Effects of Aloe Gel and Other Topical Antibacterial Agents in Rat Skin, Phytotherapy Research, Vol. 9, No. 6, pp. 455-
457.

Heggers J. P., Pineless G. R., and Robson M. C., (1979). Dermaide Aloe/Aloe vera Gel: Comparison of the Antimicro- bial Effects, The American Journal of Medical Techno- logy, Vol. 41, pp. 293-294.

Heizer W. D. (1968). Protein-Losing Gastroenteropathy and Malabsorption Associated with Factitious Diarrhea, Annals of Internal Medicine, Vol. 68, No. 4, pp. 839-852.

Hutter J.A., Salman M., Stavinoha W.B. (1996). Antiinflammatory C-glucosyl chromone from Aloe barbadensis.J Nat Prod.; 59:541–543.

Ishii Y., Tanizawa H., Takino Y. (1994). Studies of Aloe. V. Mechanism of Cathartic Effect. (4), Biological & Phar- maceutical Bulletin, Vol. 17, No. 5, pp. 651-653.

Ito S., Teradaira R., Beppu H., Obata M., Nagatsu T., Fujita K. (1993). Properties and Pharmacological Activity of Carboxypeptidase in Aloe arborescens Mill. var. Natalen- sis Berger, Phytotherapy Research, Vol. 7, No. 7, pp. S26-S29.

Jakkala LK and Sharique A. Ali. (2016). Aloe vera protects the aluminium induced changes in testicular enzymes activity of albino rats, Rattus norvegicus. World Journal of Pharmacy and Pharmaceutical Sciences Vol 5(5) 1091-1104.

Jakkala LK and Sharique A. Ali. (2016). Amelioration of the toxic effects of aluminium induced histopathological changes in testis of albino rats by Aloe vera. World Journal of Pharmacy and Pharmaceutical Sciences Vol. 5(5) 806-814.

Jensen GS, Lenninger MR, Beaman JL, Taylor R, Benson KF. (2015). Support of Joint Function, Range of Motion, and Physical Activity Levels by Consumption of a Water-Soluble Egg Membrane Hydrolyzate. J Med Food.18(9):1042-8.

Jia X., Ma X., Wei D., Dong J., Qian W. (2008). Direct formation of silver nanoparticles in cuttlebone derived organic matrix for catalytic applications.Colloids Surf A, Physicochem Eng Aspects, 30; pp. 234–240.

Joerger T. K., Joerger R., Olsson E., Granqvist C. (2001), Bacteria as workers in the living factory: metal accumulating bacteria and their potential for materials science.Trends Biotechnol, 19 pp. 15–20.

Joseph B. and Raj S. J., (2010). Pharmacognostic and Phytochemical Properties of Aloe vera Linn An Overview, International Journal of Pharmaceutical Sciences Review & Research, Vol. 4, No. 2, pp. 106-110.

Kareman E S (2013). A Self-controlled Single Blinded Clinical Trial to Evaluate Oral Lichen Planus after Topical Treatment with Aloe Vera, Journal of GHR; 2(4): 503-7.

Kaufman T., Newman A. R., Wexler M. R. (1989). Aloe vera and Burn Wound Healing, Plastic and Reconstructive Surgery, Vol. 83, No. 6, pp. 1075-1076.

Kavyashree G., George R. (2015). Aloe Vera: Its Uses in the Field Of Medicine and Dentistry, IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-ISSN: 2279-0853, p-ISSN: 2279-0861.Volume 14, Issue 10 Ver; PP 15-19

Kemp M. C., Kahlon J. B., Chinnah A. D., Carpenter R. H., McAnalley B. H., H. R. and McDaniel W. M., Shannon. (1990). In Vitro Evaluation of the Antiviral Effects of Ace- mannan on the Replication and Pathogenesis of HIV-1 and Other Enveloped Viruses: Modification of the Proces- sing of Glycoprotein Glycoprotein Precursors, Antiviral Res, Vol. 13, Suppl. 1, pp. 83.

Kim H. S., Kacew S., Lee B. M. (1999). In Vitro Chemo- preventive Effects of Plant Polysacchrides (Aloe barbadensis Miller, Lentinus edodes, Ganoderma lucidum, and Coriolus vesicolor), Carcinogenesis, Vol. 20, No. 8, pp. 1637-1640.

Krishna Kumar HN, Chandana E, Preethi SD, Chauhan JB. (2007).In vitro antimicrobial activity and phytochemical screening of Aloe vera. 4(3): 45-7

Kim H. S., Lee B. M. (1997). Inhibition of Benzopyren DNA Adduct Formation by Aloe Barbadensis Miller, Carcinogenesis, Vol. 18, No. 4, pp. 771-776.

Kokate CK. (2005). Pharmacognosy edition thirtieth published by Nirali Prakashan Pune, page 181-186.

Kumar L and Sharique A Ali. (2015). Aloe vera protects aluminium induced changes in brain enzyme activity of albino rats, Rattus norvegicus. Biosc.Bitech.Res.Comm. Vol. 8(2) 197-203.

Kumar V. P. P. N., Shameem U., Kollu P., R. L., Kalyani S. V. N. P. (2015). Green Synthesis of Copper Oxide Nanoparticles Using Aloe vera Leaf Extract and Its Antibacterial Activity against Fish Bacterial Pathogens. BioNanoScience, Volume 5, Issue 3, pp 135-139.

Kumbhar P.S., Patil1 A.B., Khopade P.S., Patil A.R., Disouza J.I. (2015). Aloe vera phytochemical constituents and medicinal properties: review. World Journal of Pharmaceutical Research Vol. 4, Issue 5, 709-728.

Kunkel G., (1984). Plants for Human Consumption,Koeltz Scientific Books.

Lad V.N., Murthy Z.V.P. (2013). Rheology of Aloe barbadensis Miller: A naturally available material of high therapeutic and nutrient value for food applications. Journal of Food Engineering Vol.115, Issue 3;279–284.

Lee J. K., Lee M. K., Yun Y. P., Kim Y., Kim J. S., Kim Y. S., Kim K., Han S. S., Lee C. K. (2001). Acemannan Purified from Aloe vera Induces Phenotypic and Functional Maturation of Immature Dendritic Cells, International of Immunopharmacology, Vol. 1, No. 7, pp. 1275- 1284.

Lee K.E., Hanafiah M.M., Halim A., Mahmud M.H. (2015). Primary Treatment of Dye Wastewater Using Aloe vera-aided Aluminium and Magnesium Hybrid Coagulants. Proceedings Environmental Sci.Vol.30; pp 56-61.

Maenthaisong, R. N., Chaiyakunapruk and Niruntra S. (2007). The Efficacy of Aloe vera for Burn Wound Heal- ing: A Systematic Review,Burns, Vol. 33, No. 6, 2007, pp. 713-718.

Maharjan H., Radha and Nampoothiri P. Laxmipriya (2015). Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review, J Tradit Complement Med; 5(1): 21–26.

Marshall J. (2000). Aloe vera Gel: What Is the Evidence. The Pharmaceutical Journal, Vol. 244, pp. 360-362.

Mehta R., Mawase S., Hasan Z.U. (2016). Antioxidant Activity of Two Year Old Aloe Vera Plants Extract After Applying of Organic Manure. Indian journal of research, Vol.5,Issue : 3;pp 152-155.

Miraj M, Jakkala L, Khan N and Ayesha S Ali. (2014). On the toxicity of certain metals and its amelioration through herbal extracts. Biosc.Biotech.Res.Comm. 7(2): 99-107.

Mitra S. K., Gopumadhavan S., Muralidhar T. S. (1996). Effect of D-400 an Ayurvedic Herbal Formulation on Experimentally Induced Diabetes Mellitus, Phytotherapy Research, Vol. 10, No. 5, pp. 433-435.

Miroddi M., Navarra M., Calapai F., Mancari F., Giofre V., Gangemi S., Calapai G. (2015). Review of Clinical Pharmacology of Aloe vera L. in the Treatment of Psoriasis. Phytotherapy Research Vol.29, Issue 5, pp. 648–655.

Moghadamtousi SZ., Kadir HA., Hassandarvish P., Tajik H., Abubakar S., Zandi K. (2014). A Review on Antibacterial, Antiviral, and Antifungal Activity of Curcumin. BioMed Research International, Article ID 186864,12 p.

Muammar Al., Elsadek M., M. F.; Shafie El, M. (2016).Potential effect of fortified pan bread with Aloe vera juice on alloxan-induced diabetic rats. African Journal of Traditional, Complementary & Alternative Medicines. 2016, Vol. 13 Issue 1, p17-24.

Mizuuchi Y., Shi S.P., Wanibuchi K. (2009). Novel type III polyketide synthases from Aloe arborescens. FEBS J;276:2391–2401.

Montaner J. S., Gill J., Singer J. (1996). Double-Blind Placebo Controlled Pilot Trial of Acemannan in Advanced Humanimmune-Deþciencyvirus Disease, Journal of Ac- quired Immune Deficiency Syndromes and Human Retro- virology, Vol. 12, No. 2, pp. 153-157.

Muller Lissner, S. A. (1993). Adverse Effects of Laxatives: Facts and Fiction, Pharmacology, Vol. 47, Suppl. 1, pp. 138-145.

Nadkerni K. M. (1976). Indian Materia Medica, 3rd Edition, Bombay Popular Prakashan Private Limited, Mumbai.

Ni Y., Turner D., Yates K.M., Tizard I. (2004). Isolation and characterization of structural components of Aloe vera L. leaf pulp. Int Immunopharmacol; 4:1745–1755.

Noor A., Gunasekaran S., Manickam A. S., Vijayalakshmi M. A. (2008). Antidiabetic Activity of Aloe vera and Histology of Organs in Streptozotocin-Induced Diabetic Rats, Current Science, Vol. 94, No. 8, pp. 1070- 1076.

Pandey A., and Singh S. (2016). Aloe vera: A Systematic Review of its Industrial and Ethno-Medicinal Efficacy. Int. J. Pharm. Res. Allied Sci., 5(1):21-33.

Pani G.K., Rath P., Maharana L., Barik R., Senapati P.K. (2016). Assessment of heavy metals and rheological characteristics of coal ash samples in presence of some selective additives. International Journal of Environmental Science and Tech.Vol. 13, Issue 2, pp 725-731.

Peng S. Y., Norman J., Curtin G., Corrier D., McDaniel H. R., Busbee D. (1991). Decreased Mortality of Norman Murine Sarcoma in Mice Treated with the Immuno modulator, Acemannon, Molecular Biotherapy, Vol. 3: pp. 79-87.

Peter A. (2002). Aloe vera Myth or Medicine Positive Health Publications.

Poor MR., Hall JE., Poor AS. (2002). Reduction in the incidence of alveolar osteitis in patients treated with Sali Cept Patch, containing acemannan hydrogel. J Oral Maxillofac Surgery 60:374-379.

Rajasekaran S., Ravi K., Sivagnanam K., Subramanian S. (2006). Beneficial effects of Aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes. Clin Exp Pharmacol Physiol; 33:232–237.

Ramachandran, Sudarshan, Rajeshwari G, Annigeri, Vijayabala GS. (2012). Aloe vera in the treatment of oral sub mucous fibrosis a preliminary study. J Oral Pathol Med. 41:755-761.

Reddy C. H., Uma, S. K., Reddy and J. Reddy. (2011). Aloe vera A Wound Healer, Asian Journal of Oral Health and Allied Sciences, Vol. 1, pp. 91-92.

Reynolds J. E. F. (1993.). Martindale, the Extra Pharmaco- poeia, 30th Edition, Pharmaceutical Press, London.

Reynolds T. and Dweck A.C. (1999). Aloe vera gel leaf, a review update. J Ethnopharmaclo 68:3-37.

Reynolds T., and Dweck, A. C. (1999). Aloe vera Leaf Gel: A Review Update, Journal of Ethnopharmacology, Vol. 68, No. 1-3, pp. 3-37.

Rezazadeh F., Moshaverinia M., Motamedifar M., Alyaseri M. (2016). Assessment of Anti HSV-1 Activity of Aloe Vera Gel Extract: an In Vitro Study. J Dent (Shiraz). 2016 Mar; 17(1): 49–54.

Rockville M. D. (1992). United States Pharmacopeia, Drug Information, United States Pharmacopeial Convention,.

Rodriguez, D. J., D., Hernández-Castillo R., Rodriguez- Garcia and Angulo-Sanchez J. L. (2005). Antifungal Activity in Vitro of Aloe vera Pulp and Liquid Fraction against Plant Pathogenic Fungi, Industrial Crops and Products, Vol. 21, No. 1, pp. 81-87.

Sadeghi B., Gholamhoseinpoor F. (2015). A study on the stability and green synthesis of silver nanoparticles using Ziziphora tenuior (Zt) extract at room temperature. Spectrochim Acta Part A: Mol Biomol Spectrosc, 134; pp. 310–315.

Sahgal A., Chaturvedi S.S., Bagde H., Agrawal P., Suruna R., Limaye M.(2015). A Randomized Control Trial to Evaluate Efficacy of Anti-bacterial and Anti-inflammatory Effect of Aloe vera, Pomegranate and Chlorhexidine Gel against Periodontopathogens. Journal of International Oral Health; 7(11):33-36.

Sahu P. K., Giri D. D., Singh R., Pandey P., Gupta S., Atul Kumar Shrivastava A.K., Ajay Kumar A., Pandey K.D.(2013).Therapeutic and Medicinal Uses of Aloe vera: A Review, Pharmacology & Pharmacy, 4; 599-610.

Salazar S. (2010). Efficacy of topical Aloe vera in patients with oral lichen planus: a randomized double-blind study, J Oral Pathol Med; 39: 735–40.

Saroj P. L., Dhandar D. G., and Singh R. S., (2004). Indian Aloe, Central Institute for Arid Horticulture, Bikaner.

Satish S. K. A., Raveesha and Janardhana G. R. (1999). Anti- bacterial Activity of Plant Extracts on Phytopathogenic xanthomonas campestris pathovars, Letters in Applied Microbiology, Vol. 28, No. 2, pp. 145-147.

Sharma P., Kharkwal A.C., Kharkwal H., Abdin M Z., Varma A.(2014). A Review on Pharmacological Properties of Aloe vera, Int. J. Pharm. Sci. Rev. Res., 29(2); Article No. 07, Pages: 31-37.

Shelton M. S. (1991). Aloe vera, Its Chemical and Therapeutic Properties, International Journal of Dermatology, Vol. 30, No. 10, pp. 679-683.

Shireen F., Manipal S., Prabu D. (2015). Anti-fungal activity of Aloe vera: In vitro study. SRM Jou. of Res. in Dental Sci. Vol. 6, Issue 2; pp91-95.

Shokri N.A., Ghasemi H.A., Taherpour K. (2016). Evaluation of Aloe vera and synbiotic as antibiotic growth promoter substitutions on performance, gut morphology, immune responses and blood constitutes of broiler chickens. Animal Science Journal, DOI: 10.1111/asj.12629.

Siegers C. P. (1993). Anthranoid Laxative Abuse A Risk for Colorectal Cancer, Gut, Vol. 34, No. 8, pp. 1099- 1101.

Siegers C. P. (1992). Anthranoid Laxatives and Colorectal Cancer, Trends in Pharmacological Sciences, Vol. 13, pp. 229-231.

Silvaa S.S., Oliveiraa M.B., Manoa J.F., Reisa R.L. (2014). Bio-inspired Aloe vera sponges for biomedical applications. Carbohydrate Polymers.Vol. 112, pp; 264–270.

Stanley M.C., Ifeanyi O.E., Eziokwu O.G. (2014). Antimicrobial effects of Aloe vera on some human pathogens. Int.J.Curr.Microbiol.App.Sci.3(3): 1022-1028.

Sundarkar P. (2011). Use of Aloe Vera in dentistry, Journal of Indian Academy of Oral Medicine and Radiolog; 23(3):
S389-91.

Surjushe A., Vasani R., Saple D. G. (2008). ALOE VERA: A SHORT REVIEW, Indian J Dermatol; 53(4): 163–166. PMC2763764.

Susman Ed. (2016). Aloe Vera Reduces Radiation-Induced Oral Side Effects. Oncology Times:Vol. 38 – Issue 6 – p 32.

Sydiskis R. J., Owen D. G., Lohr J. L,. Rosler K. H. and Blomster R. N. (1991).Inactivation of Enveloped Viruses by Anthraquinones Extracted from Plants, Antimicrobial Agents and Chemotherapy, Vol. 35, No. 12, pp. 2463-2466.

Syed, T. A., Afzal M., Ashfaq A. S. (1997). Management of Genital Herpe in Men with 0.5% Aloe vera Extracts in a Hydrophilic Cream: A Placebo-Controlled Double-Blind Study, Journal of Dermatological Treatment, Vol. 8, No. 2, pp. 99-102.

Tan Z., Li F., Xing J. (2011). Separation and purification of Aloe anthraquinones using PEG/salt aqueous two-phase system. Sep Sci Technol. 2011;46:1503–1510.

Tanaka M. (2006). Identification of Five Phytosterols from Aloe vera Gel as Antidiabetic Compounds, Biological and Pharmaceutical Bulletin, Vol. 29, No. 7, pp. 1418-1422.

Thomson R. H. (1971).Naturally Occurring Quinines, 2nd Edition, Academy Press, London.

Turner C., Williamson D.A., Stroud P.A., Talley D.J. (2004). Evaluation and comparison of commercially available Aloe vera L. Products using size exclusion chromatography with refractive index and multiangle laser light scattering detection. Int. Immuno pharmacol.; 4:1727–37.

Tyler V. E. (1994). Herbs of Choice, Pharmaceutical Products Press, New York,

Tyler V. E., Bradley L. R., Robbers J. E. (1988). Pharma- cognosy, 9th Edition, Lea & Febiger, Philadelphia.

Visuthikosol V., Chowchuen B., Sukwanarat Y., Sriurairatana S., Boonpucknavig V. (1995). Effect of Aloe vera Gel to Healing of Burn Wound a Clinical and Histologic Study, Journal of the medical Association of Thailand, Vol. 78, No. 8, pp. 403-409.

Vogler B.K., Ernst E. (1999). Aloe vera: a systemic reiview of its clinical effectiveness. British Journal of general practice 49:823-828.

West D. P., Zhu Y. F. (2003). Evaluation of Aloe vera Gel Gloves in the Treatment of Dry Skin Associated with Occupational Exposure, Vol. 31, No. 1, American Journal of Infection Control, pp. 40-42.

Yagi A., Egusa T., Arase M., Tanabe M., Tsuji H. (1997). Isolation and Characterization of the Glycoprotein Fraction with a Proliferationpromoting Activity on Human and Hamster Cells in Vitro from Aloe vera Gel, Planta Medica, Vol. 63, No. 1, pp. 18-21.

Yagi A., Kabash A., Mizuno K., Moustafa S., Khalifa M. T. I., Tsuji H. (2003). Radical Scavenging Glycoprotein Inhibiting Cyclooxygenase-2 and Thromboxane A2 Synthase from Aloe vera Gel, Planta Medica, Vol. 69, No. 3, pp. 269-271.

Yagi A., Sato Y., Miwa Y., Kabbash A., Moustafa S., Shimomura K., El-Bassuony A. (2006).Ribosomal DNA Sequence Analysis of Different Geographically Distributed Aloe vera Plants: Comparison with Clonally Regenerated Plants, Saudi Pharmaceutical Journal, Vol. 14, No. 3-4, pp. 208-211.

Yeh, G. Y., Eisenberg D. M., Kaptchuk T. J., Phillips R. S. (2003). Systematic Review of Herbs and Dietary Supplements for Glycemic Control in Diabetes, Diabetes Care, Vol. 26, No. 4, pp. 1277-1294.

Zawahry M. E., Hegazy M. R. and Helal M. (1973).Use of Aloe in Treating Leg Ulcers and Dermatoses, International Journal of Dermatology, Vol. 12, No. 1, pp. 68-73.

Zhang X., Wang H., Song Y., Nie L., Wang L., Liu B. (2006). Isolation,structure elucidation, antioxidative and immunomodulatory properties of two novel dihydrocoumarins from Aloe vera. Bioorganic & Medicinal Chemistry Letters.16, 949-53.

Rodriguez D. J., D. Castillo R H., Rodriguez G., and Sanchez J. L. A. (2005). Antifungal Activity in Vitro of Aloe vera Pulp and Liquid Fraction against Plant Pathogenic Fungi, Industrial Crops and Products, Vol. 21, No. 1, pp. 81-87.

Davis RH., Agnew PS. (2006). Antiarthritic activity of anthraquinones found in Aloe for podiatric medicine. J Am.Pod. Med. Assn. 76: 2: 61-66.

Davis RH, Leitner MG, (2012). Topical anti-inflammatory activity of Aloe vera as measured by ear swelling. J Am Pod Med Assn. 77: 11: 510-512.

Saito M., Tanaka M., Misawa E., Nabeshima K., Yamauchi K., Abe F., Yamamoto Y., Furukawa F. (2016). Oral administration of Aloe vera gel powder prevents UVB-induced decrease in skin elasticity via suppression of over expression of MMPs in hairless mice. Bioscience, Biotechnology, and Biochemistry. Vol. 80, Issue 7.

Boonyagul S., Banlunara W., Sangvanich P., Thunyakitpisa P. (2014). Effect of acemannan, an extracted polysaccharide from Aloe vera, on BMSCs proliferation, differentiation, extracellular matrix synthesis, mineralization, and bone formation in a tooth extraction model. Odontology July 2014, Volume 102, Issue 2, pp 310-317.

Cartera P.,Rahmana SM., Bhattaraia N. (2016). Facile fabrication of Aloe vera containing PCL nanofibers for barrier membrane application. Journal of Biomaterials Sci., Polymer Edition.Vol. 27, Issue 7;pp. 692-708.

Cellini L., Bartolomeo S. Di, Campli E. Di, Genovese S., Locatelli M. and Giulio M. (2014). In vitro activity of Aloe vera inner gel against Helicobacter pylori strains. Letters in Applied Microbiology Vol. 59, Issue 1, pp; 43–48.

Hajheydari Z., Saeedi M., Semnanid KM., Soltanic A. (2014). Effect of Aloe vera topical gel combined with tretinoin in treatment of mild and moderate acne vulgaris: a randomized, double-blind, prospective trial. Journal of Dermatological Treatment Volume 25, Issue 2, pp.123-129.

Haque S., Ara F, Iqbal M., Begum H., Alam NN. (2015).Effect of ethanolic extract of Aloe vera (Aloe barbadensis) gel on blood glucose level of alloxan induced hyperglycaemic mice.

Joshy K.S Sharma C.P., Kalarikkal N.K., Kumar S., Sabu Thomas S., Pothen L.A., (2016). Evaluation of in-vitro cytotoxicity and cellular uptake efficiency of zidovudine-loaded solid lipid nanoparticles modified with Aloe vera in glioma cells. Materials Science and Engineering: C Volume 66, (1); P.40–50.

Kaithwasa G., Singhb P., Bhatia D. (2014). Evaluation of in vitro and in vivo antioxidant potential of polysaccharides from Aloe vera (Aloe barbadensis Miller) gel. Drug and Chemical Toxicology Vol. 37, (2); pp. 135-143.

Kaushik R., Jain J. and Rai P. (2016). Therapeutic potentials of cow derived products- a review. IJPSR, 2016; Vol. 7(4): 1383-1390.

Mansour G., Ouda S., Shaker A., Abdallah H .M. (2013). Clinical efficacy of new Aloe vera and myrrh-based oral mucoadhesive gels in the management of minor recurrent aphthous stomatitis: a randomized, double-blind, vehicle-controlled study. Journal of Oral Pathology & Medicine Vol. 43, Issue 6, pp.405–409.

Qian Y., Yaoa J., Russelc M., Chena K., Wangd X. (2015). Characterization of green synthesized nano-formulation (ZnO–A. vera) and their antibacterial activity against pathogens. Environmental Toxicology and Pharmacology Volume 39, Issue 2, pp. 736–746.

Sahu P.K.,Giri D.D., Singh R., Pandey P., Gupta S., Shrivastava A., Kumar A., Pandey K.D. (2013).Therapeutic and Medicinal Uses of Aloe vera: A Review. Pharmacology & Pharmacy(4); 599-610.

Sayed A.M., Ezzat S.M., Khalil M.N., and Hawary S.S.(2016).Chemical composition and evaluation of possible alpha glucosidase inhibitory activity of eight Aloe species. Journal of Medicinal Plants Research. Vol. 10(13), pp. 167-178.

Shalabia M., Khilob K., Zakariac MM., Elsebaeia MG., Abdod W., Awadine W. (2015). Anticancer activity of Aloe vera and Calligonum comosum extracts separately on epatocellular carcinoma cells. Asian Pacific Journal of Tropical Biomedicine Vol. 5, Issue (5); 375–381.

Suksomboon N., Poolsup N., Punthanitisarn S. (2016). Effect of Aloe vera on glycaemic control in prediabetes and type 2 diabetes: a systematic review and meta-analysis. Journal of Clinical Pharmacy and Therapeutics Vol. 41, Issue 2, pp. 180–188.

Taukoorah U., and Mahomoodally M.F. (2016). Crude Aloe vera Gel Shows Antioxidant Propensities and Inhibits Pancreatic Lipase and Glucose Movement In Vitro. Advances in Pharma.Sci.Article ID 3720850, 9 p.

Vastrad J.V., Goudar G., , Byadgi SA., Devi RD., and Kotur R. (2015).Identification of bio-active components in leaf extracts of Aloe vera, Ocimum tenuiflorum (Tulasi) and Tinospora cordifolia (Amrutballi). Journal of Medicinal Plants Research. Vol. 9(28), pp. 764- 770, Volume 64, 1 July 2016, Pages 157–166.

Xua C., Dinga C., Zhoub N., Ruana X.M., Guoa B.X. (2016). A polysaccharide from Aloe vera L. var. Chinensis (Haw.) Berger prevents damage to human gastric epithelial cells in vitro and to rat gastric mucosa in vivo. Journal of Functional Foods Vol. 24, pp.501–512.

Yonehara A., Tanaka Y., Kulkeaw., Era T., Nakanishi Y., and Sugiyama D. (2015). Aloe vera Extract Suppresses Proliferation of Neuroblastoma Cells In Vitro Anticancer Res August 2015 35 (8) 4479-4485.

Zanuzzo F. S., Urbinati E. C.,Nash G. W., Gamperl A. K. (2015). Steelhead trout Oncorhynchus mykiss metabolic rate is affected by dietary Aloe vera inclusion but not by mounting an immune response against formalin-killed Aeromonas salmonicida. Volume 87, Issue 1.P. 43–53.

Zhou Z., Ren X. (2015) Consumption of Aloe vera Mucilage Attenuates Plasma Oxidative Stress and Dyslipidemia in Type 2 Diabetic Rats. Glob J Biotechnol Biomater Sci 1(1): 001-003.