Antibacterial activity of two extracts aqueous and methanol extract of three varieties of mango seeds was done against gram negative E coli bacterial strain. Antibacterial activity was studied by “agar disc diffusion” method. The methanolic extract of langra and aqueous extract of sindhri showed highest antibacterial activity against E coli suggesting that the phytochemicals present in these three varieties of mango can be used as an alternate source of medicine for treatment of bacterial infections. Comparative analysis has been done against three commercial antibiotics Rifampicin, Gentamicin and Apoxicillin The present study suggests that the kernel of Mangifera indica can be used as an effective potential candidate for the development of new strategies to treat bacterial infections.