Bioscience Biotechnology Research Communications

An International  Peer Reviewed Refereed Open Access Journal

P-ISSN: 0974-6455 E-ISSN: 2321-4007

Bioscience Biotechnology Research Communications

An Open Access International Journal

Mohammad Miraj1,2*, Msaad AlZhrani1, Mazen AlQahtani1, Ahmad Alanazi1, Fuzail Ahmad1,
Faizan Kashoo1, Abdul Rahim Shaik1, Mohamed Seyam1, Deepak Kumar2,3, Achal Pathak3,
Kamran Afzal4, Ahmad Abdullah A Alsaleh4, Mazen AlQahtani5 and Mehrunnisha Ahmad6

1Department of Physical Therapy and Health Rehabilitation, College of Applied
Medical Sciences, Majmaah University, AlMajmaah 11952, Saudi Arabia

2Department of Applied Health Sciences, AIHMS, New Delhi, India

3Department of Physiotherapy, Santosh University, Ghaziabad, India

4Department of Basic Sciences, College of Medicine, Majmaah University, AlMajmaah 11952, Saudi Arabia

5College of Applied Medical Sciences, Al Mareefa University, Riyadh 13713, Saudi Arabia

6Department of Nursing, College of Applied Medical Sciences, Majmaah
University, AlMajmaah 11952, Saudi Arabia

Corresponding author email:

Article Publishing History

Received: 25/10/2021

Accepted After Revision: 30/12/2021


Proprioceptive Neuromuscular Facilitation (PNF) is a popular flexibility training, which involves stretching and contraction of the target muscle group. PNF stretching is effective among athletes for improving passive range of motion, flexibility, and performance. Patients who undergo total knee replacement surgeries exhibit pain, swelling, decreased range of motion, muscle weakness, and stiffness. This study aimed to investigate the effect of the hold relax PNF technique to improve the balance and muscle strength in subjects with a bilateral knee replacement. Sixty four patients were selected based on inclusion/exclusion criteria. The patients were allocated into two groups by random allocation. The experimental group A (n=32) received the Hold Relax PNF technique with conventional treatment whereas, the control group B (n=32) received only conventional treatment as a part of the Home Exercise Program (HEP).

Outcome measures used for evaluation included the Visual Analogue Scale (VAS), Manual Muscle Testing (MMT), and Berg Balance Scale (BBS) Score for measuring pain, muscle strength, and balance respectively. Students t-test showed significant differences between variables mean scores from two groups after three weeks of intervention. There was a statistical significant (t(32) = 2.38, p = 0.02) change in the mean scores of VAS in the experimental group (mean difference pre-post=3.19) as compared to control group ( mean difference pre -post=3.19) with Cohen’s d = 2.744588. Similarly for MMT(t(32) = 0.415, p = 0.005) and BBS scores (t(32) = 2.628, p = 0.035). The result of the study suggests that the Hold Relax PNF technique is more effective than conventional treatment alone in decreasing pain as well as increasing balance and muscle strength in bilateral knee replacement patients.


PNF(Hold Relax), Knee Arthroplasty, Home Exercise Program.

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Miraj M, AlZhrani M, AlQahtani M, Alanazi A, Ahmad F, Kashoo F, Shaik A. R, Seyam M, Kumar D, Pathak A, Afzal K, Alsaleh A. A. A, Mehrunnisha Ahmad M. Effectiveness of Hold Relax Proprioceptive Neuromuscular Facilitation Technique in Total Knee Arthroplas. Biosc.Biotech.Res.Comm. 2021;14(4).

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Miraj M, AlZhrani M, AlQahtani M, Alanazi A, Ahmad F, Kashoo F, Shaik A. R, Seyam M, Kumar D, Pathak A, Afzal K, Alsaleh A. A. A, Mehrunnisha Ahmad M. Effectiveness of Hold Relax Proprioceptive Neuromuscular Facilitation Technique in Total Knee Arthroplas. Biosc.Biotech.Res.Comm. 2021;14(4). Available from: <a href=””></a>

Copyright © Miraj et al., This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use distribution and reproduction in any medium, provide the original author and source are credited.


Total knee arthroplasty (TKA) procedures have expanded dramatically in last one decade due to aging population with  rising lifestyle diseases, resulting in increased prevalence of Osteoarthritis, especially in Knees. The exponentially growing health insurance linked with increasing penetration of private  healthcare has led to changes in payment policies which has caused  post-TKA rehabilitation primarily been transferred  to home health and outpatient settings.

Knee arthroplasty is a surgery that replaces the weight-bearing surfaces of the knee joint to relieve pain and impairment, most typically been used to treat osteoarthritis apart from other knee illnesses including RA and psoriatic arthritis. Knee replacement surgery can be conducted in two ways: partial or total. A knee replacement might be more accurately termed as knee resurfacing because only the surfaces of the bone are replaced (Lauermann et al., 2014, Varacallo, Luo, and Johanson, 2021).

Whether comparing rehabilitation received in different venues or analyzing different physical therapy (PT) procedures, numerous studies have indicated that rehabilitation is related with improved post-TKA results. Following total knee arthroplasty, the mainstay of rehabilitation is achieving maximal feasible knee range of motion (ROM) and achieving specified functional goals (TKA) (Koca et al.,2014; Yang et al.,2019; Han et al.,2021). ROM is one of the most important outcomes after TKA since patients with less than 105° of knee flexion have difficulty going up and down stairs or getting out of a low chair, and is a major sign of patient discontent (Han et al.,2021).

Literature review suggests that different strategies and techniques are being used  for improving range of motion in comparison to other strategies used primarily on healthy or elderly individuals (Oh 2013; Rhyu, Kim and Park, 2015; De Carvalho et al.,2017). The Proprioceptive Neuromuscular Facilitation technique(popularly called as PNF) is one such popular technique been used to increase ROM and flexibility (Hindle et al.,2012).

Originally developed by Dr. Herman Kabat in year 1940, the technique mainly  works on the principle of raising neuro-inhibition mechanism for releasing muscular spasm and elongating muscle length, or increasing neuro-excitation mechanism for boosting muscle strength. It comprises of 3 main techniques namely, Hold Relax (HR), Contract Relax (CR), and Contract Relax Agonist Contract (CRAC)(Gonzalez, Gomez and Garcia, 2012).

The effect of PNF stretching on both joint restriction improvements is less well understood. Likewise, balance impairment, which is again one of the major problems encountered by the patients after knee replacement is due to the weakness of quadriceps muscles, which is further a consequence of the direct trauma from the surgical incision. The hold relax PNF technique is one of the most popular techniques and helps to develop muscular strength and endurance joint stability, mobility, neuromuscular control, and coordination all of which are aimed at improving the overall functional ability of people (Hindle et al., 2012; Koca et al.,2014; De Carvalho et al.,2017).

However, not many evidence-based researches are present, where the Hold Relax PNF technique has been used in patients with total knee arthroplasty(Kim Kang and Kim,2018; Han et al.,2021). Hence, the  present research study aims to explore the application of PNF stretch for changes in pain, muscle strength, and balance and therefore on the overall functionality of the lower extremity following total knee arthroplasty.


The study design was a randomized controlled trial (pretest-posttest experimental control group design), where the patients were recruited from Rehabilitation Center, College of Applied Medical Sciences, Majmaah University located at Al-Majmaah city. Necessary approval was taken from . The study was commenced in Sept 2020 and was completed in May 2021. A total of  64 subjects between the age of 42 to 73 years were included in the study who fulfilled the inclusion criteria of patients diagnosed with OA knee as well as undergoing bilateral TKR.

The exclusion Criteria included knee flexion more than 110 degrees or thigh girth more than  55 cm, poor cognition, CNS or PNS neuromuscular disease, received operation in recent 3 months, joint infection and loosening, revision total knee arthroplasty, or complication during follow up. Those who met the study’s eligibility requirements and gave their consent to participate were finally chosen to participate in the study.

The minimum sample size of  60 participants was required using statistical power of 80%, the effect size of 0.20, and a level of significance of 0.05 to measure the effectiveness of the technique used in the study. In the present study, 32 patients were recruited who fulfilled the inclusion criteria subjects were then randomly assigned into experimental  (n=32) and control groups (n=32).

While the Experimental group A(n=32) received PNF along with conventional treatment, the control group B, (n=32) received only conventional treatment. The outcome measures used for evaluation were Manual Muscle Testing (MMT) and Berg Balance Score (BBS) were used for measuring muscle strength and balance respectively.

The Hold relax PNF technique is one of the key PNF techniques. In this method, the muscle under treatment is lengthened to the point of limitation or to the extent that is comfortable for the subject.  The subject then performs a pre-stretch, end range, an isometric contraction for 5 to 10 seconds followed by voluntary relaxation of the tight muscle(Oh 2013). The Conventional treatment protocol which was the Home exercise Program(HEP) was illustrated in Table 1(Minshull et al., 2014; Kim  et al 2018).

The protocol was same as described  in an earlier published research, (Alaca et al  2015). The physiotherapist sat in front of the patient on a low-height stepper with one hand placed on the lower quadriceps muscle as well as another hand placed on the lower tibia anteriorly. Command was given to the subject to perform knee extension against resistance and was asked to hold for 6 to 10 seconds followed by relaxation as per detailed protocol given in Table 1.

Table 1. showing treatment protocol for experimental and control group.

Week 1.

  • Active quad contraction
  • Safe independent ambulation with a walker
  • Passive knee extension to 0°
  • Knee flexion to 90° or greater
  • Control of swelling, inflammation, bleeding.
Week 2.

Same as phase 1

  • Straight leg raising test
  • Hip abduction and adduction


Week 3

  • VMO recruitment during quad sets and SLR
  • Terminal knee extension 45 to 0°
  • Hamstring curls
  • Knee flexion to at least 115°


Hold Relax PNF Treatment(Group A)

  • Hold relax technique to start from Day 7 of TKR up to 3wks.
  • Muscle under Treatment (Quadriceps or hamstrings muscle)
  • Few seconds (6 – 10 second /10 repetitions) is given for quads   muscle as well as 10 repetitions is given for hamstring muscle/day
  • Patient position: Patient is sitting on high bed knee at 90 flexion
  • Back should be in a straight position
  • Foot should not touch on the ground
Group A

(Hold Relax PNF + Conventional Home Exercise Program)

Group B

(Conventional Home Exercise Program)

Statistical analysis: Using the Statistical Package of Social Science (SPSS) version 22.0, the data were checked for normality assumptions using the Kolmogorov–Smirnov test (Chicago, IL). The normally distributed data were then analyzed for mean, SDs, t-values, and p values to conclude group comparisons. The unpaired t-test score was used to compare participant characteristics and demographic data between groups.


The baseline characteristics values for the demographic characteristics for all the participants are depicted in Table 2 with no significant differences observed between the two groups in terms of baseline demographic data and clinical findings (Table 1, p≥0.05). The treatment for both groups continued for 3 weeks with readings measured for all the outcome variables at 0 weeks (Baseline line) and 3rd week (Table 3,4 and 5).

Table 2. showing baseline characteristics of the patients in both group A and B

Characteristics Group A

(n =32)

Group B

(n =32)

Gender (M: F) 22: 10  18: 14
Mean Age 65.54(7.2) 65.06(6.87)
Mean BMI 29.54(4.53) 31.22(5.88)
Baseline Active Flexion 41(11.89) 48(12.44)
Baseline Passive flexion 48(14.53) 52(13.54)
Baseline Knee Circumference 41.56(3.31) 42.74(2.94)

Table 3. showing VAS Score between group A and group B

VAS Score Group A Group B T score(p-value)
Week 1 6.68(0.54) 6.83(0.79) NS
Week 3 2.77(0.75) 4.61(0.58) 2.38(0.02*)

Visual Analogue Scale (VAS) readings were measured for both the groups at Week 1 followed by 3 weeks of intervention in both groups. It was observed that while significant improvement was observed in both groups but on intergroup comparison Group A fared better than group B(p=0.02*)(Table 3).The outcome measures of Manual Muscle Testing for both the legs for both the groups at week 1 and Week 3 are depicted in Table 4.

Regarding the MMT measurement between the two groups A and B, about both the limbs (Right and Left leg) it was observed that over the 3 weeks both groups witnessed a significant change in the muscle strength, however, group A demonstrated a greater gain in strength in both rights ( T score = 1.8 and 0.33, p<0.05**) and left leg (T-score =1.031 and 0.4, p<0.05**) respectively(Table 4).

In Table 5, within a group as well as an intergroup comparison for groups A and B were measured for the BBS scale. The table shows a statistically significant difference over the 3 weeks both within the groups (T value (Gp. A, t score = 24.04 and Gp. B=10.22, p<0.05**) as well as between the groups with Gp. A showing significant improvement in BBS as compared to Group B(t score = 2.628, p<0.05**) (Table 5).

Table 4. Showing the manual muscle testing (MMT) score for both groups A and B

MMT Group A (n=32) Group B (n=32) T score


Mean SD Mean SD
WEEK 1 Right Leg F 3 0.365 2.8*8 0.342 NS
E 2.31 0.479 2.63 0.5 NS
WEEK 3 Right Leg F 3.94 0.443 3.75 0.447 1.192(p=0.008**)
E 3.75 0.447 3.69 0.602 .333(p=0.062**)
MMT Group A n=32 Group B n=32 T score


Mean SD Mean SD
WEEK 1 Left Leg F 3.06 0.443 3.06 0.25 NS
E 2.44 0.629 2.69 0.479 NS
WEEK 3 Left Leg F 3.94 0.443 3.75 0.577 1.031(p=0.043*)
E 3.81 0.403 3.75 0.447 .415*(p=0.005**)

**significant at 0.05 level

Table 5. Showing  Berg balance scale (BBS) score for both groups A and B


(Within Group Comparison)

Group A (n=32) T-Value Group B (n=32) T score


Mean SD   Mean SD
WEEK 1 16.88 4.787 NS 16.83 4.745 NS
WEEK 3 39.06 4.739 24.04** 33.19 7.583 10.220**


Between Group Comparison
At Week 3 Group A(n=16)   Group B (n=16) T score


39.06 4.739 33.19 7.583 2.628**


** significant at 0.05 level, mean and SD of BBS (week 1 and week 3) for both groups A and B

The purpose of the present study was to investigate the effectiveness of the hold relax PNF technique to improve balance and muscle strength in subjects with a bilateral knee replacement. The result suggests that there was a significant improvement in PNF group A when compared with the conventional group B. Visual analog Scale (VAS), Manual muscle testing (MMT), and Berg Balance Score (BBS) were found to be statically significant in both the groups, however in the PNF group A, there was a significant decrease in pain along with a significant increase in MMT and BBS score than the conventional group as their mean and standard deviations suggested (p<0.05**, Table 3,4 and 5).

The results of the study draw an analogy from the study conducted by De Carvalho et al. (2017) where two exercise protocols were compared for postural balance among elderly women.  They found the PNF group showed a significant reduction in most of the stabilometric parameters. Similarly, the  BBS score in the experiment group was also found better than the control group thus concluding that women in the PNF group demonstrated better improvement in postural static stability using evaluation of various stabliometric parameters. Likewise, significant improvement was also noticed for dynamic balance using functional test performance.

It is suggested that the hold relax PNF technique more effectively increases muscle activity with decreased muscle fatigue. This could be because during hold relax PNF, isometric contraction of the muscle causes autogenic inhibition, which inhibits – motor neurons of contracted muscle and synergist in interneuron and activates antagonist by transmitting tendon tension to the anterior horn of the spinal cord via 1 b afferent nerve fiber (Minshull et al.,2014). Concentric contraction generates reciprocal inhibition, which activates -motor neurons of contracted muscles and synergists while inhibiting antagonists during agonist contraction (Cha, Cho and Choi, 2014).

The findings gain credibility by yet another study conducted by Kim, Kang, and Kim(2018) in Korea where PNF exercises were found to improve range of motion, pain, and functional activity among TKR patients. However, the present study differs from the Korean study using the outcome measures since the authors measured the improvement by objective indices including the MMT and BBS scores.

In yet another study by Rhyu, Kim, and Park (2015), a six-week elastic band exercise program using proprioceptive neuromuscular facilitation (PNF) was evaluated to measure isotonic strength of abductor’s muscles in the lower extremity. It was found that PNF-based band exercises significantly improved the peak explosive muscular power in the isotonic contraction of the lower limb abductors. It was hypothesized that the PNF facilitates certain physiological changes which potentiate the functioning of both the central and peripheral vestibular system and thereby are responsible for the therapeutic results.

The perception of articular position and mobility is critical for maintaining strength and balance as reduced articular sensitivity contributes to balance impairments in both the aged and osteoarthritic individuals (Jette et al. 2020). These imbalances are caused by decreased articular mobility. Both pain and decreased proprioception have an adverse effect on the joint increasing the risk of falling. In the present study, using hold relax PNF Technique in the experimental group, had brought out significant change(p=0.035*) in Berg Balance Score(BBS) thereby proving its efficacy post arthroplasty (Table 5). This suggest that PNF might improve both neuromuscular control as well as joint stability bring better control in both static and dynamic balance (Gong 2020).

There are some limitations to this study. The trial was limited to the Rehabilitation Department of Majmaah University and generalizability of the study cannot be justified. The study used only bilateral TKR patients and therefore evaluating the effects of the PNF techniques in unilateral cases arthroplasty especially in terms of  strength, balance and proprioception cannot be emphasized clearly.


The  data of the study conclude that the addition of the hold relax technique of PNF to the consercation exercise program decreases pain,  improves range of motion and balance in patients with bilateral TKR as compared to conservative exercise progam alone.


The authors are grateful to the Deanship of Scientific Research, AlMajmaah University via group No. RGP-2019- 35 for funding through Vice Deanship of Scientific Research Chair.

Declaration of Interest Statement :The authors report no conflicts of interest.


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