Recently, the development of antitumor drugs has been gradually transformed from cytotoxic drugs to develop new targeted drugs and low toxicity with high specificity drugs. In this study, amygdalin ZnO-Nps were synthesized by adding plant extract and exhibited as white pellet, then characterized by UV-Vis. Spectroscopy which showed an absorption peak at 261 nm, and Scanning Electron Microscope were exhibited size of the nano particle ranged between (20.00−30.26) nm and aggregated as helical shape. While Atomic Force Microscope showed the 2D and 3D of nanoparticles with size of 13.2 nm. The percentage of decreasing viability was increasedwithincreased the concentration, and the IC₅₀ values of the MCF7 and WRL cells were 121.9 and 43.6 µg ̸mL respectively. Amygdalin Zno-NPs inhibited proliferation of MCF7 and PC3 cells in a dose-dependent manner. The data demonstrate that amygdalin exerted cytotoxic effect on MCF7 as well as PC3 cells. Treatment with amygdalin induced caspase-9 activation, and induced apoptosis of cancer cells mediated by endogenous mitochondrial pathway, and the significant induction was at 200 µg/ml. In addition, it induced cell membrane permeability, cytochrome- C and total nuclear intensity significantly at 200 µg/ml.