Radiation has validated its existence and clinical utility in diagnostic and therapeutic applications. At the same time, the risks of radiation that may arise from these applications to the people working in these fields, the general public and the environment are enormous and therefore, need to be assessed and controlled effectively by improving capabilities for controlling hazards, preventing accidents, responding to emergencies and mitigating any harmful consequences of radiation. To critically evaluate the AERB guidelines against the comprehensive audit program given by IAEA for entire radiation facilities and to propose the comprehensive audit program for the institutions / universities. To design the comprehensive audit protocol for national radiation practices at institutional/University level also to propose an upgraded model for accreditation guidelines therein notified by the AERB as against the IAEA comprehensive audit protocol for radiation facilities. The comprehensive audit programs by IAEA the guidelines for the radiation safety and quality assurance by AERB,were compared and evaluated in order to propose the enhanced national audit protocol for the institutions for improving the radiation practicewhich is mainly carried out by the Medical Physicist and Radiological safety officer with the help of Licensee and Employer of the institute / universityon radiation safety and quality for the better streamlined quality and clinical services. Itwill be very useful to improve patient care with the intention of maximizing the effect of clinical care and minimizing its harm to the individual and to society as a whole also patient care, the effective use of resources, the provision and organization of clinical services, professional education and training. The optimized and justified utilization of radiation with radiation dose limitation to radiation worker and the general public in order to minimize the stochastic effect and to avoid the deterministic effect of radiation.