Most nosocomial infections reported so far have been associated with medical device based infections. A combination of opportunistic pathogens is responsible for causing implant infections. The severe form of implant infections – biofilm, has caused problems in identifying appropriate treatments because biofilm possess undeniable ability to sustain shear force and shows antimicrobial resistance. The biofilm formation is dependent on cell attachment over implant surface which can be made from variety of biomaterials. The presence of biomaterial choices for implant production can be limited down to their ability to attach cells during infection. Thus, the present study evaluates metal, ceramic, polymer and carbon based biomaterials for susceptibility towards biofilm formation. The results have shown that polymer based implants are most susceptible to biofilm formation than other metal implants and mixed implants. These results can be correlated with other in vivo analysis such as cytotoxicity to select most appropriate biomaterial for implant preparation to reduce biofilm infection and improve life of the implant.