Bioscience Biotechnology Research Communications

An Open Access International Journal

P-ISSN: 0974-6455 E-ISSN: 2321-4007

Bioscience Biotechnology Research Communications

An Open Access International Journal

Jyotsana Singh and Amar P. Garg*

School of Biological Engineering & Life Sciences, Shobhit Institute of
Engineering & Technology, Modipuram, Meerut, Uttar Pradesh, India

Corresponding author email:

Article Publishing History

Received: 16/01/2022

Accepted After Revision: 25/03/2022


Vinegar-treated eatables are widely used to improve digestion and are also known for their antimicrobial activity. The evaluated antimicrobial activity of apple cider vinegar (ACV) treated and untreated eatables-ginger (Zingiber officinale), garlic (Allium sativum), onion (Allium cepa), raw papaya (Carica papaya), white radish (Raphanus sativus) and green chilli (Capsicum annum) were analysed against selected common food borne pathogens named Escherichia coli (ATCC8739), Bacillus subtilis, Staphylococcus aureus (ATCC 6539), Shigella flexneri (ATCC 12022), Salmonella typhi (ATCC 14028), Cronobacter sakazakii (ATCC 29544), Vibrio parahaemolyticus (ATCC 17802) and V. cholera (ATCC 3906) using agar well diffusion technique. Different methods for extraction of phytochemicals have been compared. The eatables were soaked in water for 24 hours, then followed by centrifugation which yielded highest number of phytochemicals. All untreated eatables showed high to moderate antimicrobial activities against all test pathogens, while ACV-treated showed higher antimicrobial activities.


Antimicrobial activity, Apple Cider vinegar, Eatables, Extraction Methods, Phytochemicals.

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Singh J, Garg A. P. Antimicrobial Activity of Apple Cider Vinegar Treated Selected Vegetables Against Common Food Borne Bacterial Pathogens. Biosc.Biotech.Res.Comm. 2022;15(2).

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Eatables like ginger (Zingiber officinale), garlic (Allium sativum), onion (Allium cepa), white radish (Raphanus sativus), raw- papaya (Carica papaya), and green chili (Capsium annum) are commonly used in different household preparations with and without vinegars, and also in various food products within the industry. Apple cider vinegar (ACV) is known to possess a wide range of biological activities that include antimicrobial, antioxidant, anti-diabetic, anti-inflammatory, anti-hypertensive, immune-stimulatory, anticancer and others (Kalaba et al. 2019; Benedek et al. 2022). Ginger (Zingiber officinale) which belongs to the family-Zingiberaceae, is cultivated for its medicinal and spices/condiment purposes. This spice is also useful in the treatment of common cold, headache, muscular and rheumatic disorders. Ginger has a distinct spicy flavour and a pleasant aroma. The specific fragrance is attributed to its essential oil which is approximately up to 3% (Hara et al. 1998; Unuofin et al. 2021).

Gingerol is the main bioactive compound in ginger. This spice also contains zingerone, shogaol, gingerol, paradol, β-phellandrene, curcumene, cineol, geranyl acetate, terpineol, terpene, borneol, geranyl, limonene, zingiberol, linalool, α- zingiberene, β-sesquiphellandrene, β-bisabolene, zingiberenoland α-farnesene (Manuhara et al. 2018; Yan et al. 2021; Benedek et al. 2022). Ginger has high amounts of antioxidants including phenolic compounds, alanine, and vitamin C. Due to this, ginger is often used by boiling in water to be consumed as a beverage. These antioxidant compounds have an important role in maintaining human health. Besides this, the antioxidants are also widely used as food additives to prevent food damage and add value to the food (Tsai et al. 2005; Habinshuti et al. 2018; Priya et al. 2019; Yan et al. 2021). White Radish (Raphanus sativus) belongs to the family- Brassicaceae. It helps in weight loss and increases the metabolism for improved bodily processes (Gamba et al. 2021).

Similarly,  white radish helps in treatment of colon, kidney, intestinal, stomach and oral cancers, it also improves the immunity, removes mucous from throat and fight cold and cough. Onion (Allium cepa) belongs to the family- Liliaceae. Onion has recorded 6000 years BC old roots and is widely used for its several minerals and vitamins. Onion is also used as a medicine. Available research ahs shown that it is better to use raw onion because after boiling it loses its medicinal properties (Elisabetsky 1991; Sami et al. 2021; Benedek et al. 2022).

Papaya (Carica papaya) belongs to the family Caricaceae. It is a rich source of antioxidant and vitamin (A, B, C and E) and some minerals including magnesium and potassium. Papaya has medicinal properties that makes it effective against dyspepsia, hyperacidity, dysentery and constipation. It is also useful in digestion of proteins because it has high proteolytic enzymes (Dawson and Emma 1997; Parin 2020). Chilli (Capsicum annum) belongs to “Solanaceae” family and is rich in vitamins, especially vitamin C and is known for its characteristic non-pungent and pungent taste (Brito-Argaezet al. 2009; Batiha et al. 2020).

Various enteric pathogens are known to adversely affect human health and cause several intestinal diseases. However, these are constituent part of the normal gut microflora of small intestine and act as opportunistic pathogens that are responsible for wide range of infections. E. coli is a Gram’s-ve, facultative anaerobe, rod-shaped, coliform bacterium, commonly found in lower intestine of healthy people and animals. Few strains of E. coli (O157:H7) produces a powerful toxin that damages the lining of the small intestine, which can cause bloody diarrhoea (Mead et al. 1999; Braz et al. 2020; Haindongo et al. 2022).

Salmonella typhi is also a Gram-ve, rod-shaped, flagellated bacterium which causes systemic infection and typhoid fever in humans. It is transmitted through food and water. Shigella is another Gram’s -ve, facultative anaerobe, non-spore forming, non-motile, rod shaped and a leading bacterial pathogen cause of diarrhoea. The most common symptoms are fever, nausea, vomiting, stomach cramps and flatulence. It is also commonly known to cause large and painful bowel movements. Many commercial antibiotics like Azithromycin (Zithromax), Penicillin, Amoxicillin, Trimethoprim, Ciprofloxacin etc. are being used to fight against such enteric pathogens, but use of such antibiotics is not a good choice due to their high cost, multi-drug resistance and side effects e.g., amikacin and gentamycin used against E. coli leads to hearing loss, vertigo and kidney damage (Lewis et al. 2012; Haindongo et al. 2022).

The aim of this study was to determine the antimicrobial activity of untreated and  apple cider vinegar treated ginger (Zingiber officinale), garlic (Allium sativum), onion (Allium cepa), white radish (Raphanus sativus), raw-papaya (Carica papaya) and green chilli (Capsicum annum) extracts on different pathogenic bacteria to find out their beneficial uses and to carry out their phytochemical screening of the extracts so as to evaluate the impact of vinegar treatment on their antimicrobial activities.


All eatables were collected from the local market of Meerut (Uttar Pradesh) India and washed well to clean the soil particles, peeled off and were further washed thoroughly again with clean water. After washing, these were dried under sunlight for 2-3 days and 5 g of each material was soaked in 25 mL of distilled water and 25 mL of apple cider vinegar (ACV) separately for one week. After soaking, these were crushed. Phytochemical analysis and antimicrobial activities were evaluated.

For the physical evaluation of eatables, Evaporable Moisture Content and Total Ash Content were calculated. Evaporable Moisture Content was determined by subtracting the fresh weight from sun dried mass. For Total Ash Content, weighed number of dried eatables was heated at 550°C temperature for 6 h in muffle furnace and the ash content was determined using following formulae:

Different methods were used for extraction of phytochemicals from ginger and finally the best was followed for rest eatables. For Soxhlet Method, 5 g dry powder of ginger was dissolved/suspended in 500 mL of distilled water and kept at 70°Cfor 6 to 7 h to evaporate the water, the pellet was dissolved in sterile distilled water to make final volume of 25 mL.

For Heating Method with Filtration, 5g powder of ginger was dissolved/suspended in 25mL of distilled water and heated till boiled, mixed properly and filtered through normal filter paper, final volume was adjusted to 25mL. For Heat without Filtration: 5g powder of ginger was dissolved/suspended in 25 mL of distilled water and heated till boiled, kept for 2 h and just decanted, final volume of extract was adjusted to 25 mL. During Simple Soaking followed by Centrifugation, 5g ginger powder was soaked in 15 mL of distilled water over night and was simply centrifuged at 2000rpm for 15 min.

The extraction procedure was repeated 3 times, each time with 3 mL water and finally all extracts were mixed and final volume of 25 mL was made. Besides this, the antimicrobial activity of different eatables with or without apple cider vinegar (ACV) was evaluated using agar well diffusion method on Müller Hinton agar. The plates were incubated in an upright position at 37±1℃ for 24 to 48 h. and the diameter of zone of inhibition (in mm) was measured against  test  human pathogens. Lastly, Qualitative standard chemical tests were carried out for phytochemical screening of untreated and ACV- treated eatables using AOAS protocol as described (Trease and Evans 1978; Rawat and Garg 2021).


Moisture evaporated by sundry method revealed that sun dry weight of garlic was highest followed by onion, green chili and ginger (Fig 1) while the ash content of ginger was highest followed by garlic, white radish and onion. It shows that ginger, garlic and white radish contain high amounts of inorganic minerals. Chili had lowest ash content (1.2% only) which suggests that it has lesser mineral contents but its other contents contributes to medicinal and digestive value (Batiha et al. 2020). White radish had highest evaporable moisture content showing its greater and easy availability during digestion (Gamba et al. 2021). On comparison of phytochemical composition of ginger extracted by Soxhlet method, heating with and without filtration, and simple soaking followed by centrifugation, it was found that latter method yielded highest amount of all phytochemicals followed by heating without filtration and Soxhlet method showed poor results (Table1) (Gamba et al. 2021).

It may be due to the loss of vital phytochemicals by evaporation during Soxhlet procedure. Overnight soaking followed by centrifugation avoided the heat treatment which allowed greater release/leaching of phytochemicals. It, was therefore, suggested that overnight soaking followed by centrifugation should be used for phytochemical analysis of biomaterials. Each phytochemical was analyzed using two different tests to ensure their presence or absence in untreated and ACV- treated eatables and the results (Table 2, Fig. 2) revealed that ACV- treated ginger showed tannins; onion and raw papaya possessed terpenoids and green chili gave positive test for saponins while these phytochemicals were absent in  the untreated vegetables.

It was also found that no phytochemical was lost due to apple cider vinegar treatment and ACV treatment improved the nutritive value of the vegetables (Benedek et al. 2022).

To assess the antimicrobial activity of untreated and ACV- treated eatables, agar well diffusion assay was used against Escherichia  coli, Pseudomonas aeruginosa, Staphyloccus aureus, Shigella flexneri, Salmonella typhi, Cronobacter sakazakii, Vibrio parahaemolyticus, Bacillus subtilis and Vibrio cholera (Table 3, Fig. 3, 4) and comparison of their antimicrobial activity showed that ACV-treated ginger should not be used if suffering from typhoid fever as its treatment reduced antimicrobial activity of ginger against Salmonella typhi (Table 3). ACV-treated green chili either reduced or showed little effects on antimicrobial activity of almost all vegetables.  It suggests that green chilies should not be eaten after treatment with apple cider vinegar. ACV-treated white radish and raw papaya generally enhanced antimicrobial activity against all test species except Pseudomonas aeruginosa where it showed little or no effects. ACV-treated onion showed reduced antimicrobial activity against E. coli which suggests that apple cider vinegar-treated onion should not be eaten by a person suffering from colitis.

The presence of medicinally active constitutes like flavonoids, alkaloids, saponin, tannins, anthraquinones, terpenoids and glycosides in untreated and ACV-treated eatables determines their nutritive value and antimicrobial activity (Manuhara et al. 2018; Benedek et al. 2022).

The present study shows that the extracts of eatables possess antimicrobial compounds and are  beneficial for health. Vinegars are commonly used as food condiment and preservatives. Apple cider vinegar is also used in the Ayurvedic pharmaceutical industry because of its medicinal properties. Ginger is well known for its antimicrobial activity and is widely used in Ayurveda for various treatments and also in food industry for flavor and aroma (Rajsekhar 2012; Kalhoro et al. 2022). Antibacterial activity of some leafy vegetables has been reported in previous studies against S. aureus, S. pyogenes, B. subtilis, E. coli and P. aeuginosa (Bhat and Al-Daihan 2014; Mahendranathan and Abhayarathne 2021).

Fruits and vegetable are also known for their antimicrobial activity and papaya, potato, cucumber, beet root and ginger have been found to inhibit E. coli, S. aureus, Lactobacillus, and Proteus vulgaris (Narinder et al. 2017; Kumar et al. 2021). Antimicrobial activity of fermented vegetable byproducts of some vegetable has been evaluated in a previous study which showed that fermented extracts of tomato, melon and carrot possessed higher antimicrobial activity than commercial preservatives against Salmonella spp., Lysteria monocytogenes and B. cereus (Ricci et al. 2021).

Hossaini et al. (2020) have evaluated antimicrobial effects of medically relevant green leafy vegetables and have found that ethanolic and methanolic extracts of Azadiracta indica, Coccinia grandis, Ipomoea aquatica and Paederia foetida leaves extracts possess antimicrobial activity against Staphylococcus spp., Klebsiella spp., and Pseudomonas spp., while crude and hot water extract showed almost no effect on bacterial growth (Hossaini et al. 2020; Ricci et al. 2021). Antimicrobial activity of vinegar is attributed to the phenolics, organic acids, microbial metabolites of the fermenter organism as well as its high acid component and has been found effective against E. coli, P. aeruginosa, S. aureus using agar well diffusion technique (Yagnik et al. 2018; Ousaaid et al. 2021; Benedek et al. 2022).

Antimicrobial activity of ginger extract against P. aeruginosa, S. aureus, Proteus mirabilis, E. coli, B. subtilis and S. typhi has been demonstrated in previous studies (Akintobi et al. 2013; Unuofin et al. 2021). In view of the increasing demand of natural products with health promoting attributes, the antimicrobial activity of grape vinegars against S. aureus, E. coli and Candida albicans is reported in the previous study (Antoniewicz et al. 2021). Our results reveal that apple cidar vinegar -treated white radish and raw papaya should be used while ACV-treated ginger should be avoided when suffering from typhoid fever and similarly ACV-treated onion should not be consumed in colitis. Green chili should not be treated with ACV at all and should be used raw (Benedek et al. 2022).

Figure 1: Percent moisture, sun dry and ash contents of eatables used.

Figure 2: Qualitative phytochemical tests for analysis of various groups from eatables

Table 1. Comparison of different extraction methods for estimation of various phytochemical groups using ginger as a model.

Extraction methods                                          Phytochemical tests
Flavonoid Alkaloid Tannins Saponins Anthraquinone Terpenoid Glycoside
Soxhlet method + +   _ ++ _ + ++
Heat filtration method ++ + _ + _ ++ +++
Heat without filtration method +++ ++ _ +++ _ ++ +
Maceration method ++ + _ ++ _ + ++
Centrifuge method ++++ +++++ _ +++++ _ ++++ +++++

Table 2. Phytochemical analysis of various biomolecules from apple cider vinegar (ACV) treated
and untreated eatable in water extract (WE) using centrifugation method.



Eatable’s extract
   Ginger    Garlic   Onion Raw papaya Green         chilli White radish
Alkaloids + + + + + + + + + + + +
Saponins + + + + + + + + + + +
Tannins + + + + + + + + + + +
 Flavonoids + + + + + + + + + + + +
Anthraquinones + + + + + + + +
Terpenoids + + + + + + + + + +
Glycosides + + + + + + + + + + + +

Table 3.
Zone of inhibition (mm in diam.) exhibited by water extracts (WE) of and apple cider vinegar (ACV) treated eatable’s extracts.

Test pathogen


        Garlic Ginger Onion White radish Green chilli Raw papaya
Escherichia coli 9.5 10 7.5 9 7.7 8 10 13.5 9 10.5 9 10.5
Pseudomonas aeruginosa 7.0 7.5 7.75 10 9.5 11.25 8.25 7 6.0 7.25 9 10.25
Staphylococcus aureus 6.5 7 8.25 10.75 7.72 12.25 9.0 11.25 8 8.5 7 7
Shigella flexneri  9.0 11.25 9.25 12 10.25 12 7.0 12.25 7.94 9.25 8.5 11.75
Salmonella typhi 9.5 12 7.57 9.75 7.0 11.25 8 8.25 7.68 9.75 8.5 8
Cronobacter sakazakii 10.25 10.75 7.5 7.75 8.75 7.75 10.65 12.25 7 7.75 9 9.75
Vibrio parahaemolyticus 8.5 11.75 9 10.75 7.5 12 8.8 8.5 8.23 8.25 9 12
Bacillus subtilis 8.5 9 9 9 8.25 8.25 7.89 9.5 7.5 9.25 9 9.25
Vibrio cholera 7 8.75 6.5 9.5 6 10.5 8.25 7.75 6 8.25 8 9.25

Figure 3: Zone of inhibition of water extract of different eatables: ginger (Gi), garlic (Ga), raw papaya (Rp) and onion (O) against Vibrio parahaemolyticus plate A; Shigella flexneri plate B; Pseudomonas aeruginosa plate C and Bacillus subtilis plate D.

Figure 4: Zone of inhibition of Apple cider vinegar extract of different eatables: ginger, garlic, raw papaya and onion against Pseudomonas aeruginosa plate A, Vibrio parahaemolyticus plate B, Salmonella typhi plate C and Bacillus subtilis plate D and Va denotes apple cider vinegar used as control.


The findings of the present study have concluded that overnight soaking followed by centrifugation yields better quality of phytochemicals. Ginger, garlic, onion, raw papaya, green chili and white radish possess high antimicrobial activities against Escherichia coli, Pseudomonas aeruginosa, Staphyloccus aureus, Shigella flexneri, Salmonella typhi, Cronobacter sakazakii, Vibrio parahaemolyticus, Bacillus subtilis and Vibrio cholera and their treatments with apple cidar vinegar improves their antimicrobial activity. ACV- treated white radish and raw papaya should be used while ACV-treated ginger should be avoided  in typhoid fever. ACV-treated onion should not be consumed in colitis. Green chili should not be treated with ACV and should be used raw.


The study was financially supported by the Shobhit Institute of Engineering & Technology (Deemed to-be- University), Modipuram, Meerut (UP) India. Also, the research grade pure vinegar was provided by the firm Vigour of Village Natural Vinegar, Village Food Products, Saradhana, Meerut, Uttar Pradesh.

Conflict of Interests: Authors declare no conflict of interests to disclose.


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