Hospital acquired infections specifically urinary tract infections have been commonly observed in developing countries. Urinary tract infections account for one fourth of hospital acquired infections. The patients’ exposure to pathogens has been mostly linked with their stay in critical units of health care centers. The other most common cause of these infections has been compromised immune system of patients. The present study focuses on evaluating other factors that can cause UTIs. The results show prevalence of four bacterial species – S. aureus, P. aeriginosa, K. pneumoniae and E. fecalis in clinical samples of urinary catheter, urine bags, patients’ blood and urine specimens. The study also evaluates role of catheter maintenance in causing UTIs and it has been found that hygienic practices such as cleaning of urine bag at proper time and disinfection of catheter after urination can help in controlling UTIs.  This study provides a perspective of finding unconventional sources responsible for UTI which can help in prevention of catheter based infections in future.