Antimicrobial resistance (AMR) is growing at an alarming rate leading towards becoming a threat to global health and development. According to WHO, AMR is one of the top ten global threats. Approximately 3 million antibiotic resistant infections occur in US annually with more than 35,000 deaths each year. The situation has attracted the attention of researchers towards searching for novel antibiotics and antimicrobial compounds. The present study is based on testing amino acids and their structures as novel therapy to target bacterial infections. it has been found that hydrophobic amino acids can be potential antibacterial compounds with mechanism of action based on bacterial cell membrane. The study provides low cost antibacterial compound that can be used as coating material or antimicrobial therapy for hospital setting to prevent AMR nosocomial infections in future.