Bioscience Biotechnology Research Communications

An International  Peer Reviewed Refereed Open Access Journal

P-ISSN: 0974-6455 E-ISSN: 2321-4007

Bioscience Biotechnology Research Communications

An Open Access International Journal

Rajashree Jena1, 2, Chiranjiv Pradhan3, Nikhil Kumar Maiti2 and Prasanta Kumar Choudhury1,2*

1School of Agricultural and Bioengineering, Centurion University of Technology and Management, Odisha, India

2Microbiology Unit, ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar-751002, Odisha, India

3Department of Aquaculture, Kerala University of Fisheries and Ocean Studies, Panangad, Kochi, Kerala, India

Corresponding author email: choudhury.microbio@gmail.com

DOI:

Article Publishing History

Received: 13/10/2020

Accepted After Revision: 29/12/2020

ABSTRACT:

In the current investigation efforts have been made to study the effect of Bacillus spp. on different water quality parameter in in vitro. A total of 16 (RJ1 to RJ16) bacterial strains comprising of B. subtilis (n=08), B. cereus (06) and, B. pumilis (02) were subjected to grow in synthetic pond water to evaluate the nutrient cycling and utilization pattern with respect to ammonia, nitrite, nitrate and phosphorus verses their growth rate for a 16 hrs study individually. After incubation the strains showed luxuriant growth in the media with turbidity increasing upto 12 hrs and after 14hrs stationary growth was observed in most of the isolates. All the strains showed increase in ammonia concentration (NH4-N). Highest ammonia concentration up to 50% level was observed with RJ15 and remaining isolates also increased ammonium ion concentration with the lowest range producer was RJ3 with 4%. All the strains showed nitrate reduction activity.

Strain RJ12 reduced nitrate (NO3-N) concentration (30%) from 12.04 to 8.37 mg/L. RJ5 was the lowest nitrate reducer with activity of 2%. Six strains showed reduction in nitrite concentration including RJ1 and RJ5 where the highest nitrite reduction with 38% activity was observed. All other isolates showed increased in nitrite concentration and highest was observed with RJ11 (47%). In phosphorus metabolism RJ2 was found to be utilize highest phosphorus in synthetic pond water with 32% decrease in phosphorus concentration whereas most of the strains showed no change in activity. This study showed that Bacillus spp. are ammonia producers whereas they have the potential to reduce nitrate, nitrite and utilize phosphorous in water environment.

KEYWORDS:

Ammonia, Bacillus Spp., Nitrate, Nitrite, Phosphorous

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