Environmental pollution by toxic metal has accelerated dramatically since the beginning of the industrial revolution. The primary source of this pollution include the burning of fossils, mining and smelting of metaliferous ores, municipal waste, fertilizers, pesticide and sewage. Toxic metal contamination of ground water and soil, which poses major environmental and human health problems, is currently in need of an effective and affordable technological solution. In this study, germination was conducted in Til (Sesamum orientale L.) in order to find out the effect of Ni toxicity on its germination, growth and biochemical parameters. The seeds were germinated in six different concentrations of nickel chlorode solution having 0-50 mg/l of nickel. The pot culture experiment was done with different concentrations (10, 20, 30, 40 and 50 ppm) of nickel. It was noted that the Seedling vigour index, Metal tolerance index were found to be reduced and the percentage of phytotoxicity was increased and biochemical parameters showed a declining trend with increasing Ni concentrations. The seedlings treated with Ni showed decreased chlorophyll and soluble protein content as compared to control while increased proline content was observed as compared to control.