Biorefinery Sequential Extraction of Alginate by Conventional and Hydrothermal Fucoidan from the Brown Alga, Sargassum cristaefolium
1,2Departement of Fisheries Science, Faculty of Agriculture, Madura Islamic University, Pamekasan 69351, Indonesia
Corresponding author email: email@example.com
ARTICLE PUBLISHING HISTORY
Brown algae has a fucoidan and alginate bioactive components with different characteristics. Brown algae has a great potential as feedstock for biorefinery alginate and fucoidan extraction which is integrated. Alkaline extraction process parameters in the sequential fucoidan and alginate extraction are integrated affected to the characteristics of alginate and fucoidan from brown alga Sargassum cristaefolium. This study aims to understand the effect of the parameters of the alkaline extraction process on multiple responses alginate and fucoidan yields, and determine the optimal alkaline extraction process in the integrated alginate and fucoidan sequential extraction processes according to the concept of industrial biorefinery. Box Bhenken Design from the response surface method was used to understand the effect of process parameters temperature, time and Na2CO3 levels on the multiple responses alginate (yield, intrinsic viscosity, molecular weight) and fucoidan yield. The results showed that the alkaline extraction process parameters significantly affected on the multiple responses alginate and fucoidan yield with quadratic pattern. The optimal conditions occur at a temperature of 57.02 oC, time of 123.96 min, and Na2CO3 concentration of 2.66%. Under the optimal point, the yield of alginate was 33.93%, intrinsic viscosity was 404.73 ml/g, molecular weight of alginate was 191.38 kDa and fucoidan yield was 1.92%.