Debashis Halder and Shyamapada Mandal
BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS INSIGHTS INTO THE ANTAGONISM OF
LACTOBACILLUS FERMENTUM
CURD ISOLATE 467
range: 22 – 34 mm), and intermediately susceptible to
Cx and Tm with ZDIs of 16 and 18 mm (ZDI criteria
range: 16 – 20 mm); the LAB, while, showed resistance
(ZDI: ≤15 mm) to Ak, Cp, Km, Me and Vm. Thus, the
isolated LAB, in this study, has been found to be safe,
on the basis of lack of transferable antibiotic resistance
property (Ammor etal., 2008; Imperial and Ibana, 2016),
and this LAB might be useful in single-strain based pro-
biotic formulation bene ting a large number of local
population, in this part of the globe. It has been reported
that multi-strain/multi-general probiotics might exhibit
limited functional property for universal usage, requir-
ing probiotics alternatives development and/or person-
alized probiotic approaches (Zmora et al., 2018; Suez
etal., 2018).
CONCLUSION
The Lactobacillus fermentum LMEM22, which was iso-
lated from locally available commercial curd, showed
antagonistic activity against gram-positive as well as
gram-negative pathogenic bacteria, with overall bacte-
riocin activity (AU/ml) of 142.27 – 240.00, and the ‘R’
value of 5.5 – 13.5, and (based upon the report available
in literatures, too) there is no risk of transferable antibi-
otic resistance in the LAB. Thus, the isolated LAB might
be useful as broad spectrum antibacterial biotherapeu-
tics, and such native LAB isolate might be consumed
alone, in place of antibiotic therapy, or can be used
(based upon the antibiogram of the native LAB) in pro-
biotic-antibiotic combination therapy. However, further
studies are needed to validate the probiotic attributes of
the isolated LAB, including its molecular identity as well
as the antibiotic resistance management.
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