Bioscience Biotechnology Research Communications

An Open Access International Journal

P-ISSN: 0974-6455 E-ISSN: 2321-4007

Bioscience Biotechnology Research Communications

An Open Access International Journal

Nawres Adnan Abdulameer

Technical Institute of Al-Diwaniyah, Al-Furat Al-Awsat Technical University (ATU)  Iraq.

Corresponding author email: dw.noras@atu.edu.iq

Article Publishing History

Received: 12/05/2021

Accepted After Revision: 14/07/2021

ABSTRACT:

The present study was conducted for the purpose of verifying the effect of silver nanomaterial and comparing it with mint leaf extract on male rats treated with ethidium bromide. Where 50 male rats were selected and divided into 5 groups as follows:Control group 10 males rats gavage distilled water for 30 days, Ethidium bromide group 10 males rats gavage distilled water for 30 days, Ethidium bromide and mint leaf extract(40g/L) group 10 males rats for 30 days, Ethidium bromide and silver nanoparticle group 10 males rats for 30 days, Ethidium bromide and (silver nanoparticle, mint leaf extract) group 10 males rats for 30 days.And after sacrificed males rats we collected tissues samples from testis to extraction of RNA  to evaluated of LH,FSH and testosterone by qPCR technique and tissues to make tissues section to knowledge changes in testis. The results of the current study, which was conducted on male rats, showed that there is a positive effect of mint leaves extract as well as a negative effect of silver nanoparticles on the level of gene expression for all study groups in each of the LH hormone, FSH hormone and testosterone compared to the control group as shown in the Fig 1,2 and 3. And showed in figure 4, several normal seminiferous tubules with normal spermatogonia cell, normal Sertoli cells, normal germ cell layer with normal maturation stages; interstitial spaces show an area of interstitial congestion; the lumen devoid of spermatozoa with interstitial congestion; normal seminiferous tubules with normal spermatogonia cell, normal Sertoli cells, and normal germ cell layer; maturation arrest of tubules and mild interstitial congestion; seminiferous tubules with atrophy exhibiting thick double cell layers indicative of cessation of spermatogenesis.

KEYWORDS:

Mint Leave, Silver Nanoparticle, LH, FSH And Testosterone.

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