Parveen Qureshi
774 BIOCHEMICAL ALTERATIONS DUE TO CARBARYL EXPOSURE BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS
Table 1. Effect of Carbaryl (0.04 PPM) on glucose content in liver of Clarias batrachus
S. No. Time
Amount of glucose
% Age change
(increase/decrease)
‘t’ value Probability
Control (mg) Treated (mg)
1 7 5.068 ± 0.2051 7.282 ± 0.4090 43.685879 4.3276 ≤ 0.01
2 15 5.192 ± 0.2514 4.854 ± 0.3529 18.4024 2.3212 ≤ 0.02
3 30 5.212 ± 0.3457 3.254 ± 0.1959 37.5671 4.4164 ≤ 0.01
Value expressed as mg/100mg wet-weight of tissue
Each value is the mean ± standard error of 5 individual observations.
Table 2. Effect of Carbaryl (0.04PPM) on glucose content in alimentary canal of Clarias batrachus
S. No. Time
Amount of glucose
% Age change
(increase/decrease)
‘t’ value Probability
Control (mg) Treated (mg)
1 7 0.392± 0.032 0.541 ± 0.0448 37.500 -2.41921 ≤ 0.05
2 15 0.408 ± 0.0511 0.322 ± 0.026 -21.0784 1.2837 ≤0.10
3 30 0.411 ± 0.051 0.286 ± 0.0308 -28.713861 1.7247 ≤ 0.10
and Singh 2010). When pesticides pass over from water
into other links of biological chain, their content grows
hundreds and thousands of times. Besides observed by
ltering organisms, persistent poisonous chemicals may
be deposited in the tissues and then get into the organ-
ism of a sh in the interconnected links of food chains,
the action of the pesticides, being cumulative is ampli-
ed several times, (Omoniyi 2018).
The main stream of the earlier toxicological studies
on the pesticides on shes was con ned to histopatho-
logical eld. The effect of pollutants on tissue systems
of sh have shown to produce gross structural changes
such as atrophy, hyper trophy, necrosis, haemorrhage,
liqui cation, cytoplasmic vacuolation and degeneration
of blood vessels, (Mekkawy et al. 2016). Histological
changes and histopathological studies pressurized scien-
tists to go in for biochemical changes. Several scientists
have paid signi cant contribution in the histochemical
and biochemical eld (Ahmad et al. 2015). The present
attempt has been made to investigate the biological
change in the Clarias batrachus induced by sublethal
dose of carbaryl. The alimentary canals and liver were
selected for the present study because digestion, absorp-
tion and metabolism are cumulatively responsible for
energy production.
The amount of glucose in alimentary canal and liver
of Clarias batrachus was measured by Anthrone method
(Nicholas et al. 1956). About 100mg of tissue sample
was homogenized in 5 mL of chilled de-ionized water.
Then centrifuged at 5000rpm for 10min the nal volume
of supernatant was noted. Soon after the supernatant
collection, 1mL of 0.01% Sodium uoride solution was
added to supernatant to stop the conversion of glucose
to lactic acid. From the supernatant, 0.01mL was taken
as test sample. The volume in the test tube was made up
to 1mL with DDW. Then de-proteinization was done by
mixing 0.5mL of 1% H
2
SO
4
to 1mL of test sample. Then
4mL of anthrone reagent was added slowly with constant
stirring. The tubes were dipped in chilled water during
the mixing of anthrone reagent. The mixture was kept in
boiling water bath for 4min and then cooled. Develop-
ment of green color indicated the presence of glucose in
the supernatant. The intensity of the color was read at
on 540nm on spectrophotometer. Each experiment was
repeated 5times and the mean value with standard error
were calculated.
Carbohydrate, protein, lipid, enzymes and vitamins
are important component of the body and play a vital
role in the body construction, metabolism and detoxi-
cation. Therefore, in present investigation biochemi-
cal changes in glucose content have been studied in
liver and alimentary canal of control and intoxicated
Clarias batrachus on interval of 7-15-30 days. In the
present investigation an initial increase in glucose was
observed, which may be due to the greater absorption of
glucose by the intestine, under created stress conditions
and acceleration of glycogenolysis and gluconeogenesis,
which is similar to the ndings of Sharma et al. (2012).
These process synthesize glucose which is the major fuel
for energy production and energy demand of animal
increase to face the toxicity stress. Similar observations
were also made by (Singh and Singh 2017).
Besides glucose, they also observed increase of lac-
tate dehydrogenase (LDH) activity which elevates the
amount of lactic acid. These ndings are supported by
Michael (2018), as they have also reported an increase
LDH activity. Ahmad et al 2015 also reported the sig-
ni cant increased value in blood glucose and signi cant
decrease value in serum total protein level. Mekkawy
et al. (2016) also observed a decrease in LDH activity
which indicates pyruvate not dehydrogenated to yield
acetyl CoA and converted into lactic acid due to the ele-