Biotechnological
Communication
Biosci. Biotech. Res. Comm. 10(3): 415-418 (2017)
In uence of different spacing and cultivars on yield
components and biochemical parameters of onion
(
Allium cepa L.
)
Saurabh Kishor, R. B. Ram, M.L. Meena*, Sachin Kishor, D. C. Meena and Anil Kumar
Department of Applied Plant Science (Horticulture), Babasaheb Bhimrao Ambedkar University, Vidya Vihar
Rae Bareli Road Lucknow (UP), India
ABSTRACT
The investigation was undertaken to determine the effect of spacing and cultivars on economic horticultural traits of
onion. In trail different spacing was taken 7.5 10 cm, 10 10 cm, 12.5 10 cm and 15 10 cm. Three varieties viz.
Agrifound Light Red, NHRDF Red-3 and NHRDF L-28 were used for study. The layout of experimental  eld was laid
down in Factorial Randomized Block Design with 3 replications. It is clearly revealed that the signi cantly contrary,
yield ha
-1
was the highest (404.14 q ha
-1
) at closer spacing (10 10 cm) and the lowest was (362.47 q ha
-1
) at wider
spacing 15 10 cm. The weight of individual bulb of onion (49.54 g) was increased with the wider spacing (12.5 10
cm). The bulb length (6.63 cm), diameter (6.97 cm) and number of scale per bulb (8.00) also the same trend in widest
spacing (15 10 cm). The interaction of spacing and different cultivars had in uenced signi cantly on total solu-
ble solids and ascorbic acid of onion bulb. Signi cant effect was found among the varieties for total soluble solids,
ascorbic acid, reducing sugar. Studied highest total soluble solids (13.960Brix), ascorbic acid (10.03), reducing sugar
(8.50), non reducing (9.70%) and total sugars (17.70%) respectively.
KEY WORDS: ONION, SPACING, CULTIVARS, GROWTH AND YIELD
415
ARTICLE INFORMATION:
*Corresponding Author: drmeena1997@gmail.com
Received 1
st
July, 2017
Accepted after revision 12
th
Sep, 2017
BBRC Print ISSN: 0974-6455
Online ISSN: 2321-4007 CODEN: USA BBRCBA
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NAAS Journal Score 2017: 4.31 Cosmos IF: 4.006
© A Society of Science and Nature Publication, 2017. All rights
reserved.
Online Contents Available at:
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DOI: 10.21786/bbrc/10.3/13
416 INFLUENCE OF DIFFERENT SPACING AND CULTIVARS ON YIELD COMPONENTS BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS
Saurabh Kishor et al.
INTRODUCTION
Onion is a bulbous herbaceous biennial vegetable crop
which belongs to family Alliaceae and widely grown as
with cross-pollinated and monocotyledonous behavior
having diploid chromosomes 2n=16 (Bassett, 1986).
Onion is the most important bulb crop cultivated com-
mercially in most parts of the world. The crop is grown
for consumption both in the green states as well as in
mature bulbs. It is valued for its bulbs having character-
istics odour,  avor, and pungency, which is due to the
presence of a volatile oil-allyl-propyl-disulphide, (Kan-
tona etal., 2003 and Habtamu etal., 2016 and Kishor
etal., 2017).
Onion is the richest source of  avonoids in the
human diet and  avonoid consumption has been asso-
ciated with a reduced risk of cancer, heart disease and
diabetes. In addition it is known for anti bacterial, anti-
viral, anti-allergenic and anti in ammatory potential.
Even though the crop has great contribution both in
economic and health issues, its production and produc-
tivity is not scaled to the required level. This is because
use of appropriate agronomic management practices
and improved technology inputs are still not highly
used which have undoubted contribution in increasing
crop yield potential. One of the important measures to
be taken in increasing the productivity of onion is deter-
mining spacing for each agro-ecology since full package
of information is required for each growing region the
country to optimize onion productivity (Gupta, et al.,
1994). Proper spacing ensures optimum plant growth
through adequate utilization of moisture, light, spacing
and nutrients (Zubeldia and Gases, 1977). The control of
plant spacing is one of the cultural practices to control
bulb size, shape and yield (Geremew et al., 2010). The
higher yield and better control of over or under bulb
size bulb size could be obtained if plants are grown at
optimum density. Total bulb yield can be increased as
population density increase (Kantona et al., 2003 and
Habtamu etal., 2016 and Kishor etal., 2017).
Several researchers in many countries have shown
that varieties and plant spacing had profound effects
on the growth and yield of onion (Pandey etal., 1991;
Bhonden et al., 1995 and Kumar et al., 1998, Kishor
et al., 2017). Considering the above stated situations,
the present study was undertaken to determine the effect
of spacing on growth and yield of different cultivars of
onion under Lucknow conditions (Allium cepa l.).
MATERIAL AND METHODS
The experiment was conducted at Horticulture Research
Farm-II of Babasaheb Bhimrao Ambedkar University,
Vidya Vihar Rae Bareli Road Lucknow (UP) during
November 2015 to April 2016 under subtropical condi-
tion. The experiment site lies about 260 56 N latitude
and 800 52 E longitude at an altitude of 111 m above sea
level. The area experiences rainfall that stretches from
April to October with the main rainy season from June
to early September. The area receives average rainfall
between 800-1000 mm with annual and maximum tem-
perature ranging from 15 to 300C. Three varieties viz.
Agrifound Light Red, NHRDF Red-3 and NHRDF L-28
and four plant spacing such as 7.5 10 cm, 10 10
cm, 12.5 10 cm and 15 10 cm were used for study.
The experiment was laid out in Factorial Randomized
Block Design (RBD) and replicated in thrice. Standard
analytical methods were followed for recording vari-
ous parameters. The observation was made on the fol-
lowing parameters yield kg/plot, yield kg/ha, weight of
bulb, bulb length (cm), bulb diameter (cm), number of
scale per bulb, total soluble solids (0Brix) was measured
with the help of an Erma hand refractometer and were
corrected using standard reference table and express in
terms of (0Brix) at 200, ascorbic acid (mg/100g) Ascor-
bic acid content was determined by diluting the known
volume of juice with 3% meta-phosphoric acid and
titrating with 2,6- dichlorophenol-indo-phenol solu-
tion, reducing sugar (%), non reducing sugar (%) and
total sugars (% ) were determined by titrating the sample
against Fehlings solution using methylene blue as an
indicator. All the parameters were collected from  ve
randomly selected plants of each treatment. On set of
the Rabi season these healthy bulb uniform shape and
size were selected and transplanted well prepared  eld
Statistical analysis of the data obtained in different set
of experiments was calculated following the standard
procedure as stated by (Panse and Sukhatme, 1989).
RESULT AND DISCUSSION
YIELD COMPONENTS
The results obtained during the investigation in respect
to yield components parameters viz., yield kg/plot, yield
kg/ha, weight of bulb, bulb length (cm), bulb diameter
(cm), number of scale per bulb, total soluble solids (
0
Brix),
ascorbic acid (mg/100g), reducing sugar (%), non reduc-
ing sugar (%) and total sugars (% ) Table 1. The interac-
tion effect of spacing and different cultivars had in u-
enced signi cantly on the parameters. The maximum
yield (7.34 kg/plot and 489.77 q ha
-1
) was recorded from
the variety Agrifound Light Red with spacing 10 10 cm
followed by the variety NHRDF Red-3 (6.88 kg/plot and
458.77 q ha
-1
) with spacing 7.5 10 cm. The minimum
yield (4.67 kg/plot and 311.44 q ha
-1
) was recorded from
the variety Agrifound Light Red with spacing 12.5 10
cm. While the heaviest bulb (57.60 g) was recorded from
BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS INFLUENCE OF DIFFERENT SPACING AND CULTIVARS ON YIELD COMPONENTS 417
Saurabh Kishor et al.
Table 1. In uence of different spacing and cultivars on yield components and biochemical parameters f Onion
Varieties Spacing
Yield
(kg/plot)
Yield
(q/ha)
Weight of
bulb (g)
Bulb diameter
(cm)
Length of
bulb (cm)
Number of
scale per bulb
Total soluble
solids (
0
Brix)
Ascorbic acid
(mg/100g)
Reducing
sugar (%)
Non reducing
sugar (%)
Total
sugars (%)
Agrifoun Light Red (7.5x10cm)
6.59 439.55 33.22 5.01 4.86 7.00 10.66 7.43 5.50 8.47 13.97
Agrifoun Light Red (10x10cm)
7.34 489.77 36.00 5.05 5.40 7.00 10.76 8.60 6.63 8.37 15.00
Agrifoun Light Red (12.5x10cm)
4.67 311.44 22.56 5.16 5.08 7.33 12.33 8.90 6.27 8.67 14.93
Agrifoun Light Red (15x10cm)
5.81 387.33 38.20 6.86 6.86 8.00 12.53 8.83 7.93 9.77 17.70
NHRDF Red-3 (7.5x10cm)
6.88 458.77 44.33 5.49 5.40 7.00 12.50 8.83 7.73 8.83 16.57
NHRDF Red-3 (10x10cm)
6.47 431.77 40.36 5.48 5.36 7.33 13.96 8.43 7.37 8.67 16.03
NHRDF Red-3 (12.5x10cm)
5.83 388.88 48.13 5.68 5.03 7.33 12.76 9.20 7.83 8.57 16.40
NHRDF Red-3 (15x10cm)
6.71 447.33 55.43 7.06 6.53 7.66 12.06 9.93 8.23 8.67 16.90
NHRDF L-28 (7.5x10cm)
5.40 360.22 43.63 5.80 5.38 7.00 13.50 9.13 7.13 9.60 16.73
NHRDF L-28 (10x10cm)
6.14 409.55 49.80 6.52 5.10 7.33 13.23 8.40 7.60 9.00 16.60
NHRDF L-28 (12.5x10cm)
5.80 387.11 57.60 6.14 5.59 7.33 12.26 8.76 7.63 9.07 16.70
NHRDF L-28 (15x10cm)
5.66 377.77 55.00 7.00 6.51 8.33 11.86 10.03 8.50 8.97 17.47
CD (P=0.05)
0.19 12.86 8.54 0.48 N/S N/S 0.80 0.82 N/S N/S N/S
SE(m)±
0.06 4.35 2.89 0.16 0.23 0.37 0.27 0.27 0.56 0.38 0.67
the variety NHRDF L-28 with spacing 12.5 10 cm fol-
lowed by variety NHRDF L-28 with spacing 15 10 cm
(55.00 g) and lightest bulb was obtained from the variety
Agrifound Light Red with 7.5 10 cm. While the biggest
bulb diameter (7.06 cm) was recorded from the variety
NHRDF Red-3 with 15 10 cm followed by the variety
Agrifound Light Red with spacing 15 10 cm (6.86 cm).
The lowest bulb diameter (5.01 cm) was recorded from
the variety Agrifound Light Red with 7.5 10 cm. While
the length of bulb and number of scale per bulb was not
signi cantly in uenced by the different spacing and cul-
tivars. However, the highest length of bulb (6.86 cm) was
recorded from the variety Agrifound Light Red with spac-
ing 15 10 cm followed by variety NHRDF Red-3 with
15x10cm cm (6.53 cm) and the lowest bulb length (4.86
cm) was recorded from the variety Agrifound Light Red
with spacing 7.5 10 cm. Thus, the number of scale per
bulb (8.33) was increased from the variety NHRDF L-28
with spacing 15 10 cm followed by variety Agrifound
Light Red with spacing 15 10 cm (8.00). This is due to
proper spacing ensures optimum growth and weight of
bulb through adequate utilization of moisture, light, spac-
ing and nutrients (Zubeldia and Gases, 1977 Habtamu
et al., 2016 ). These results are conformity with (Kumar
etal., 1998), the highest yield with a spacing of 15 10
cm, (Kantona etal., 2003) and (Khan et al., 2003) total
bulb yield can be increased as population density increase
and (Gupta and Gaffer, 1980) bulb size and bulb weight
decreased with the decrease in spacing (Kishor etal., 2017).
Table 1 indicated that interaction of spacing and dif-
ferent cultivars had in uenced signi cantly on total
soluble solids and ascorbic acid of onion bulb. The max-
imum total soluble solids (13.96
0
Brix) were obtained
from variety NHRDF Red-3 with spacing 10 10 cm
followed by variety NHRDF L-28 with spacing 7.5 10
cm (13.50
0
Brix) and minimum (10.66
0
Brix) was recorded
from variety Agrifound Light Red with spacing 7.5 10
cm. While ascorbic acid (9.93 mg/100g) was increased
from variety NHRDF RED-3 with spacing 15 10 cm
followed by variety NHRDF RED-3 with spacing 12.5
10 cm (9.20 mg/100g) and minimum (7.43 mg/100g)
amount of ascorbic acid was recorded from variety Agri-
found Light Red with spacing 7.5 10 cm.
Thus reducing sugar, non reducing sugar and total
sugars were not signi cantly in uenced by the different
spacing and cultivars interaction. However, the maximum
reducing sugar (8.50%) was recorded from variety NHRDF
L-28 with spacing 15 10 cm followed by variety NHRDF
Red-3 with spacing 15 10 cm (8.23%) and minimum
(5.50%) was recorded from variety Agrifound Light Red
with spacing 7.5 10 cm. While maximum non reduc-
ing (9.70%) and total sugars (17.70%) were recorded from
variety Agrifound Light Red with spacing 15 10 cm fol-
lowed by spacing 7.5 10 cm and 15 10 cm with variety
418 INFLUENCE OF DIFFERENT SPACING AND CULTIVARS ON YIELD COMPONENTS BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS
Saurabh Kishor et al.
NHRDF L-28. This result is in agreement with the  ndings
of (Gupta and Gaffer 1980), (Khan etal., 2003) in onion
and (Kumar etal., 1998) obtained the better quality with
spacing of 15x10 cm in onion.
CONCLUSION
Our study was concerted to the combined application
of different spacing and varieties favorably in uenced
plant growth attributes. Results clearly emphasized the
importance of spacing as well as selection of varieties
of onion, as the conjoint use of them yielded higher and
gave a remunerative return. Based on the trend of yield
and economical aspects of onion observed in the present
study; it was concluded that for getting higher bulb yield
of onion, combined application of 10 10 cm spacing
with var. Agrifound Light Red, was best under Lucknow
conditions by 7.5 10 cm spacing with var. NHRDF
Red-2 and 15 10 cm spacing with var. NHRDF Red-2.
These results however need to be further con rmed on
multi locations large scale trials before passing as rec-
ommendations to the onion growers of Lucknow.
ACKNOWLEDGEMENT
The authors express their thanks to Director, NHRDF
Regional Station, Deoria (UP) for providing seed materials.
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