Zoological
Communication
Biosci. Biotech. Res. Comm. 10(2): 63-67 (2017)
On a new tapeworm,
Circumoncobothrium govindii
of fresh water sh
Channa marulius
from Godavari
basin India
Fartade AM and R.S. Chati
Department of Zoology, Shri Shivaji Mahavidyalaya, Shivaji Nagar, Barshi, 413411 India
ABSTRACT
The present communication deals with the description of a new species of genus Circumoncobothrium govindii Sp.
Nov., from fresh water sh Channa marulius from Godavari basin provided new data on their morphology. The
present worm differ from the known species of the genus in the shape and size of the scolex, number of hooks and
arrangement of rostellum, shape of segment, number of testes, position of cirrus pouch and arrangement of vitellaria.
KEY WORDS:
CIRCUMONCOBOTHRIUM GOVINDII
, CHANNA MARULIUS, GODAVARI BASIN
63
ARTICLE INFORMATION:
*Corresponding Author:
Received 9
th
Feb, 2017
Accepted after revision 2
nd
June, 2017
BBRC Print ISSN: 0974-6455
Online ISSN: 2321-4007 CODEN: USA BBRCBA
Thomson Reuters ISI ESC and Crossref Indexed Journal
NAAS Journal Score 2017: 4.31 Cosmos IF : 4.006
© A Society of Science and Nature Publication, 2017. All rights
reserved.
Online Contents Available at: http//www.bbrc.in/
INTRODUCTION
The genus Circumoncobothrium was erected by Shinde
(1968) from the intestine of fresh water sh Ophio-
cephalu sleucopunctatus as a type species C. ophio-
cephali Jadhav and Shinde, (1976) added three new
species of this genus viz., C. aurangabadensis and C.
raoiifrom Mastacembelus armatus and C. gachuai from
Ophiocephalus gachua. Chincholikar and Shinde, (1976 )
described two new species of this genus C. shindei from
fresh water sh Mastacembelus armatus and C. bagari-
usi from Bagarius species. Shinde, (1977) reported C.
khami from Ophiocephalusstriatus. Jadhav et.al, (1990)
described C. yamaguti, from Mastacembelus armatus
Shinde et.al. (1994) reported C. alii from Mastacembelus
armatus.Patil et al, (1998) added C. vadgaonensis as a
new species to this genus from Mastacembelus armatus.
Wongasawad and Jadhav, 1998 added C. baimaii from
Mastacembelus armatus.C. punctatusi was added by
Kalse and Shinde, 1999 from Ophiocephalus punctatus.
Shinde et. al., 2002 described C. mastacembalusae as a
new species from Mastacembelu sarmatus.Pawaret. al.,
2002 reported C. armatusae (minor)) from Mastacembelu
sarmatus to this genus. Tat and Jadhav, 2004 reported
C. manjari from Ophiocephalus gachua. Supugade et. al.,
2005 added C.vitellariensisfrom Mastacembelus arma-
64 ON A NEW TAPEWORM,
CIRCUMONCOBOTHRIUM GOVINDII
OF FRESH WATER FISH
CHANNA MARULIUS
BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS
Fartade and Chati
tus. Kharade et al., 2007 added C. cirrihinae from Cir-
rihinamrigala. Shelke et al., 2007 added C. mehdii from
Mastacembelusarmatus. Pardeshi et al., 2007 added C.
ambajogaiensis from Mastacembelus armatus.Jawalikar
et al., 2008 added C. yogeshwari from Mastacembelus
armatus. Borde and Sushil Jawale, 2008 added C. purnae
from Mastacembelus armatus. Kalse et al., 2009 added
C. naidui from Mastacembelus armatus. Shah, 2010
added C. paaddeithenensis from Mastacembelus arma-
tus. Menkudale and Jawale, 2010 added C. thapari from
Ophiocephalus stratus. Pardeshi and Hiware, 2011 added
C. jadhavae from Mastacembelus armatus. Lastly Dhole
and Kadam, 2011 added C. clariase from Clariasbatra-
chus.
MATERIAL AND METHODS
The present specimens were recorded from the intestine
of the freshly killed fresh water sh Channa marulius
(Hamilton 1822) from Godavari Basin during the period
of June 2009-May 2011. Each sh was dissected and
examined in all parts like ns, gills, scales, and vis-
ceral organs under a microscope. Fishes were opened
up dorso-ventrally and the internal organs examined.
The entire digestive system was removed and placed in
a Petri dish with physiological saline. Infection of each
group of parasites was treated as follows: collected para-
sites were rst relaxed and then xed in hot 4% formalin
and stain using Harris haematoxyline. Stained parasites
were washed in distilled water, dehydrated in ascending
grades ofalcohol, cleared in xylene, mounted in D.P.X.
Drawings were made using a camera lucida. Identi ca-
tion was carried out by using system Helminthum vol.
II, (Yamaguti, 1956)
Eight mature specimens were collected from the
intestine of fresh water sh Channamarulius (Hamilton
1822) from Paithan Dist- Aurangabad in the month of
March 2010.The parasite were attened, preserved in
4% formalin, stained with Harris haematoxylin, passed
through various alcoholic grades, cleared in xylene,
mounted in D.P.X. Whole mount slides were prepared
for further anatomical studies. Drawing was made with
the aid of Camera Lucida. All measurements are given
in millimeters. All the cestodes are long, consisting of
scolex, immature, mature and gravid proglottids. The
scolex large well developed conical in shape which
measures 7.15(7.05-7.24) in length and 4.06(3.54-4.57)
in breadth, the anterior end of the scolex terminates in
a rostellum; the rosetellum is armed with 58-60 hooks
and arranged in semicircle. The scolex bears two large
bothria, which are extended anterior to posterior end,
posteriorly it is globular and measures 4.53(4.45-4.62)
in length and 0.90(0.82-0.99) in breadth.
Neck is present but short measures 1.62(1.33-1.90) in
length and 2.80(2.74-2.86) in breadth. Mature segment
small, rectangular broader than long measures 0.74(0.69-
0.79) in length and 2.88(2.70-3.06) in breadth. The testes
oval to rounded 50-59 in numbers, measures 0.04(0.03-
0.06) in length, and 0.02(0.016-0.024) in breadth, spread
in the segment.The cirrus pouch is oval, medium in size,
anterior to ovary and measures 0.049(0.033-0.066) in
length, and 0.14 in breadth. The cirrus is thin tube meas-
ures 0.264 (0.23-0.29) in length and 0.02(0.016-0.024) in
breadth.Ovary is irregularly bilobed with long isthmus,
each lobe is different in shape, large measures 1.22(1.18-
1.25) in length and 0.099(0.066-0.13) in breadth situated
in the middle of the segment. The vagina is thin coiled
tube, starts from genital pore, posterior to cirrus pouch
and measures 0.115(0.099-0.132) in length and 0.04 in
breadth. Genital pore small, rounded and measures 0.06
in length and breadth.The gravid segment are small, rec-
tangular lled with uterus. The uterus is saccular, lled
with numerous egg and measures 0.18(0.11-0.25) in
length and 0.40(0.31-0.45) in breadth. The eggs are oval
in shape.The vitellaria are granular and arranged in two
rows at each side of the segment.
The genus Circumoncobothrium was established by
Shinde in 1968 as a type species C. ophiocephali from
Ophiocephalus leucopunctatus. The present worm comes
closer to all the known species of the genus Circumonco-
bothrium Shinde, 1968 in general topography of organs.
But differs due to some characters from followingspe-
cies. The present cestode differs from C. ophiocephal-
iShinde,(1968) in having distinct scolex, broad in the
middle and tapering at both the ends, rostellar hooks
80 in numbers, presence of neck, ovary compact, sin-
gle conical mass, vitellaria follicular and reported from
Ophiocephalus leucopunctatus, in India.
The present worm differs from C. aurangabadensis-
Jadhav and Shinde,(1976) in having the scolex broad
in the middle and narrow at both the ends, hooks 42 in
numbers, presence of neck and testes 135-145 in num-
bers. The present tapeworm differs from C. raoii Jadhav
and Shinde,(1976) in having scolex broad in the mid-
dle and narrow at both the ends, hooks 46 in numbers,
arranged in single circle, neck present,testes 210-215 in
numbers.
The present parasite differs from C. gachua Jadhav
and Shinde,(1976) in having the scolex pear shaped,
hooks 46 in numbers, neck present, mature proglottid
squarish, testes 375-400 in numbers, vitellaria follicular,
arranged in two rows and reported from Ophiocephalus
gachua, in India. The present tapeworm distinguish from
C. shinde Chincholikar and Shinde,(1976) in having the
scolex narrow anteriorly and broad posteriorly, hooks
49 in numbers, neck present, testes 260-275 in numbers,
evenly distributed and ovary dumb-bell shaped.
BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS ON A NEW TAPEWORM,
CIRCUMONCOBOTHRIUM GOVINDII
OF FRESH WATER FISH
CHANNA MARULIUS
65
Fartade and Chati
The present worm differs from C. bagariusi Chincho-
likar and Shinde,(1976) in having the scolex narrow ante-
riorly and broad posteriorly, hooks 55 in numbers, testes
275-285 in numbers, arranged in two lateral elds, vitel-
laria follicular and reported from Bagarius sp., in India.
The present parasite differs from C. khami Shinde, (1977)
in having the scolex cylindrical, hooks 48 in numbers,
lancet shaped, mature proglottidssquarish, testes 190-
200 in numbers, evenly distributed, vitellaria follicular
and reported from Ophiocephalus sp.,in India.The present
cestode differs from C. yamaguti Jadhav et.al, (1990) in
having the scolex distinct, narrow anteriorly and broad
posteriorly and testes 130-150 in numbers.
The present worm differs from C. alii Shinde et. al.,
(1994) in having scolex triangular, hooks 34 in num-
bers, neck present and testes 230-240 in numbers. The
present tapeworm differs from C. vadgaonensis Patil
et.al., (1998) in having the scolex triangular, hooks 56 in
numbers, neck present, testes 490-510 in numbers and
vitellaria follicular. The present cestode differs from C.
baimaii Wongsawad and Jadhav, (1998) in having the
scolex pear shaped, hooks 48 in numbers, neck present,
testes 88-100 in numbers, ovary compact and reported
from Masta cembelusarmatus in Chang Mai.
The present worm differs from C. punctatusi Kalse and
Shinde,(1999) in having scolex rectangular, hooks 40-50
in numbers, neck present, mature proglottids squarish,
testes 140-150 in numbers, vitellaria follicular, arranged
in 3-6 rows and reported from Ophiocephalus punctatus,
in India. The present worm differs from C. armatusae
Shinde et. al., (1999) in having scolex triangular, hooks
58 in numbers, neck present, testes 90-100 in numbers,
ovary compact and vitellaria follicular, arranged in 3-4
rows on lateral side of the segments. The present parasite
differs from C. mastacembelusae Shinde et. al., (2002) in
having scolex pear shaped hooks 30 in numbers, testes
130-140 in numbers, ovary compact and vitellaria fol-
licular, arranged in 2-3 rows on each lateral side. The
present cestode differs from C.armatusae (minor) Pawar
et. al., (2002) in having scolex triangular, hooks 58 in
numbers, testes 190-200 in numbers and vitellaria fol-
licular. The present form differs from C. manjari Tat and
Jadhav,(2004) in having the scolex triangular, hooks 48
in numbers, in single circle, neck present, testes 128-
145 in numbers, vitellaria follicular and reported from
Ophiocephalusgachua, in India.
The present parasite differs from C. vitellariensis
Supugade et. al., (2005) in having scolex large, trian-
gular, hook 48 in numbers, testes 250-260 in numbers
and vitellaria follicular, arranged in 3-4 rows.The pre-
sent parasite differs from C. cirrhinae Kharade et al.,
(2007) in having scolex large, cylindrical, barrel shaped,
hooks 56, rostellar, neck short, testes 300-305, medium,
oval, ovary dumbbell shaped, medium. The present para-
site differs from C. mehdii Shelke et al.( 2007) in having
hooks 56 arranged in single circle, neck short, squar-
ish, mature segment medium, squarish, testes 280-290
medium, ovary large, distinctly bilobed, vitellaria fol-
licular, 3-4 rows. The present cestode differs from C.
ambajogaiensis Pardeshi et al., (2007) in having hooks
18-20 in numbers, neck absent, mature segment ten time
broader than long, testes 250-300 in numbers, ovary
bilobed, dumbbell shaped, vitellaria follicular. The pre-
sent worm differs from C.yogeshwari Jawalikar et al.,
(2008) in having hooks 53 in numbers, neck very short,
testes 95-98 in numbers, vitellaria follicular, arranged
in two rows. The present worm differs from C. purnae,
Borde and Jawale, (2008) in having hooks 52 in num-
bers, neck absent, mature segment squarish, slightly
broader than long, testes 230-235 in numbers, ovary
bilobed and vitellaria follicular, arranged in 3-4 rows.
The present parasite differs from C. naidui Kalse et al.,
(2009) in having scolex cylindrical, hooks 40 in num-
bers, neck absent, testes 200-210 in numbers, medium
rounded, ovary oval, single mass, compact, transversely
elongated with acini.The present cestode differs from
C. paithenensis Shah, (2010) in having scolex triangu-
lar, cylindrical, hooks 58, single circle in four quadrant,
neck very short, mature segment two time broader than
long, testes 70-80, oval, and vitellaria follicular in two
rows.The present form differs from C. thapari Menkudale
and Jawale (2010) in having host Ophiocephalus stratus,
hooks 52 in numbers, neck absent, testes 95 in numbers,
medium, oval, ovary medium, lobed, vitellaria follicular
2-3 rows.The present parasite differs from C. jadhavae
Pardeshi and Hiware, (2011) in having scolex triangu-
lar, dome shaped, hook 35-45 in numbers, neck present,
mature segment broader than long, testes 95-105 oval
to round, ovary bilobed, vitellaria follicular, arranged
in 2 rows.The present worm differs from C.clariasiK.N
Kadam and Jaywant Dhole (2011), having scolex trian-
gular, hooks 48 in numbers, testes oval in shape 249-
259 in numbers, vitellaria follicular arranged 2-3 rows.
Reported from Clariasbatrachus in India.
Key to the species of the genus
Circumoncobothrium
(Shinde, 1968)
Neck present - 1
Neck absent - 2
1) Vitellaria granular - 3
Vitellaria follicular - 4
2) Mature segment squarish - 5
Mature segment broader - 6
than long
3) Scolex conical - C. govindiiSp.Nov
Scolex triangular - C. alli, Shinde et. al.
1994
Scolex pear shaped - C. baimaii, Wongaswad
et. al. 1988
66 ON A NEW TAPEWORM,
CIRCUMONCOBOTHRIUM GOVINDII
OF FRESH WATER FISH
CHANNA MARULIUS
BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS
Fartade and Chati
Scolex narrow anteriorly - C. Shindeii, Shinde et. al.
broad Posteriorly 1977
Scolex broad in the middle - 7
narrow at both end
Scolex cylindrical - C. cirrihinae, Kharade
et. al. 2007
4) Mature proglottids - 8
squarish
Mature proglottids - 9
broader than long
5) Testes 150-200 in - C. khami, Shinde, et. al
numbers 1968
Testes above 200 in - C. purnae, Borde and
numbers Jawale 2008
6) Hooks below 30 - C. ambajogainsis,
Pardeshi 2011
Hooks in between 30-40 - C. mastacembelusae,
Shinde 2002
Hooks in between 40-50 - 10
Hooks above 50 in number - 11
7) Testes below 200 - C. aurangabadensis,
Jadhav. et.al 1976 in
number
Testes above 200 in number - C. raoii, Shinde. et.al.
1976
8) Scolex rectangular in shape - C. punctatusi, Kalse
2009
Scolex pear shaped - C. gachuaiJadhav 1980
Scolex triangular - C. mehdii, Shelke 2007
9) Hooks 20-30 in numbers - C. armatusae, Shinde
1999
Hooks 30-50 in numbers - 12
Hooks 50-60 in numbers - 13
Hooks 60-70 in numbers - 14
Hooks 80 in numbers - C. ophiocephali, Shinde,
et.al 1968
10) Scolex triangular - C. vitellariansis,
Supugade, 2005
Scolex cylindrical - C. naidui, Kalse et. al.
2009
11) Testes in between 90-100- C. thapari, Menkudale,
2010
Testes in between 100-150 - C. yamaguti, Jadhav.
1990
Testes in between 150-200 - C. armatusae, Pawar
2002
BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS ON A NEW TAPEWORM,
CIRCUMONCOBOTHRIUM GOVINDII
OF FRESH WATER FISH
CHANNA MARULIUS
67
Fartade and Chati
Testes above 200 in numbers - C. bagariusi,
Chincholikar, 1997
12) Testes in between 90-110 - C. jadhavae, Pardeshi,
2011
Testes in between 125-150 - C. Manjari, Tat, 2004
Testes in between 250-260 - C. clariasi, Kadam 2011
13) Testes in between 70-80 - C. paithenesis, Shah
2010
Testes in between 90-100 - C. yogeshwari,
Jawalikar 2008.
Testes in between 490-510 - C. vadgaonensis, Patil
1998
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